A large scale in situ hybridization screen is actually performed in order to characterize all the genes whose expression is spatially regulated during the zebrafish embryogenesis. This in situ screen: 1. Provides a large collection of cell and/or tissue specific molecular markers usable for the analysis of the wild-type development (establishment of a """"""""molecular anatomy"""""""" of the zebrafish embryo) as well as for the phenotypic description of mutants obtained from genetic screens. 2. Provides starting points for a functional analysis (using overexpression studies by RNA injection). 3. Provides a powerful way to clone genes disrupted in mutant embryos based on the correlation between the mutant phenotypes, the expression pattern, sequence and map position of these genes. 4. All these expression data will be released to public in an integrated database (ZFIN) in a standardized format allowing search both by name, sequence, genetic map, anatomical structures and will include a description using key words of all spatially restricted expression patterns as well as the corresponding pictures for all analyzed developmental stages.
| Krug 2nd, Randall G; Clark, Karl J (2015) Elucidating cannabinoid biology in zebrafish (Danio rerio). Gene 570:168-79 |
| Yao, Zizhen; Farr 3rd, Gist H; Tapscott, Stephen J et al. (2013) Pbx and Prdm1a transcription factors differentially regulate subsets of the fast skeletal muscle program in zebrafish. Biol Open 2:546-55 |
| Fernando, Carol A; Conrad, Patricia A; Bartels, Cynthia F et al. (2010) Temporal and spatial expression of CCN genes in zebrafish. Dev Dyn 239:1755-67 |
| Maves, Lisa; Tyler, Ashlee; Moens, Cecilia B et al. (2009) Pbx acts with Hand2 in early myocardial differentiation. Dev Biol 333:409-18 |
| Walters, Kevin B; Dodd, M Ernest; Mathias, Jonathan R et al. (2009) Muscle degeneration and leukocyte infiltration caused by mutation of zebrafish Fad24. Dev Dyn 238:86-99 |
| Galloway, Jenna L; Wingert, Rebecca A; Thisse, Christine et al. (2008) Combinatorial regulation of novel erythroid gene expression in zebrafish. Exp Hematol 36:424-32 |
| Thisse, Christine; Thisse, Bernard (2008) High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat Protoc 3:59-69 |
| Mathias, Jonathan R; Dodd, M Ernest; Walters, Kevin B et al. (2007) Live imaging of chronic inflammation caused by mutation of zebrafish Hai1. J Cell Sci 120:3372-83 |
| Wingert, Rebecca A; Selleck, Rori; Yu, Jing et al. (2007) The cdx genes and retinoic acid control the positioning and segmentation of the zebrafish pronephros. PLoS Genet 3:1922-38 |
| Maves, Lisa; Waskiewicz, Andrew Jan; Paul, Biswajit et al. (2007) Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation. Development 134:3371-82 |
Showing the most recent 10 out of 29 publications
