The research goal is to develop ion mobility-mass spectrometry (IM-MS) for high-throughput, proteomic scale applications. This proposal provides plans for developing IM-MS methods with enhanced throughput, sensitivity, resolution and information content to address analytical challenges of proteomics. The research is divided into sections that address the design of new instrumentation, incorporation of high-repetition rate (kHz) lasers to increase the IM-MS duty cycle to approximately 100%, which will increase sample throughput and reduce analysis time, and development of tandem MS techniques for simultaneous acquisition of peptide mass maps and peptide sequencing for rapid protein identification. The proposal also describes research collaborations with chemical-biology research groups that have immediate needs for proteomics-mass spectrometry. Such collaborations provide critical evaluations for the technique developmental research. These collaborations include drug design studies and structure determinations of key proteins involved in protein-drug interactions, viral protein-host protein interactions as well as membrane protein trafficking to the inner nuclear membrane, studies of how cells function by identifying expressed proteins and proteins components of multi-subunit complexes in E. coil, understanding bacterial pathogenesis by determining the proteins present on the surface of the bacteria, identifying the protein composition of seminal plasma fluids of fertile and sub-fertile stallions as well as degrees of post-translational modifications and key interacting protein and protein complexes, de novo sequencing of novel neuropeptides isolated from single neurons insects and other organisms, identifying functional state of ciracadian clock proteins and large hetero-multimeric protein complex call the """"""""clockosome.""""""""

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
5R01RR019587-02
Application #
6876091
Study Section
Special Emphasis Panel (ZRG1-BECM (01))
Program Officer
Sheeley, Douglas
Project Start
2004-04-01
Project End
2007-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
2
Fiscal Year
2005
Total Cost
$218,250
Indirect Cost
Name
Texas A&M University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
078592789
City
College Station
State
TX
Country
United States
Zip Code
77845
Fernandez-Lima, Francisco A; Blase, Ryan C; Russell, David H (2010) A Study of Ion-Neutral Collision Cross Section Values for Low Charge States of Peptides, Proteins, and Peptide/Protein Complexes. Int J Mass Spectrom 298:111-118
McLean, Janel R; McLean, John A; Wu, Zhaoxiang et al. (2010) Factors that influence helical preferences for singly charged gas-phase peptide ions: the effects of multiple potential charge-carrying sites. J Phys Chem B 114:809-16
Cologna, Stephanie M; Russell, William K; Lim, Peniel J et al. (2010) Combining isoelectric point-based fractionation, liquid chromatography and mass spectrometry to improve peptide detection and protein identification. J Am Soc Mass Spectrom 21:1612-9
Williams, Brad J; Russell, William K; Russell, David H (2010) High-throughput method for on-target performic acid oxidation of MALDI-deposited samples. J Mass Spectrom 45:157-66
Tao, Lei; Dahl, David B; PĂ©rez, Lisa M et al. (2009) The contributions of molecular framework to IMS collision cross-sections of gas-phase peptide ions. J Am Soc Mass Spectrom 20:1593-602
Tao, Lei; McLean, Janel R; McLean, John A et al. (2007) A collision cross-section database of singly-charged peptide ions. J Am Soc Mass Spectrom 18:1232-8
Ranjith-Kumar, C T; Miller, William; Xiong, Jin et al. (2007) Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain. J Biol Chem 282:7668-78
Predel, Reinhard; Russell, William K; Neupert, Susanne et al. (2006) Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.). Peptides 27:2670-7
Sanchez, Elda E; Galan, Jacob A; Russell, William K et al. (2006) Isolation and characterization of two disintegrins inhibiting ADP-induced human platelet aggregation from the venom of Crotalus scutulatus scutulatus (Mohave Rattlesnake). Toxicol Appl Pharmacol 212:59-68
Nachman, Ronald J; Russell, William K; Coast, Geoffrey M et al. (2006) Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry. Peptides 27:521-6

Showing the most recent 10 out of 17 publications