Heavy drinking during pregnancy is the cause of fetal alcohol syndrome, the leading known cause of mental retardation. Even moderate drinking during pregnancy can result in a spectrum of more subtle effects which are determined by both the dose of ethanol and the gestational age at the time of exposure. It is estimated that 1% of all newborns are affected by prenatal ethanol exposure. Identifying these alcohol exposed newborns is difficult. Currently there is no laboratory tool for their identification. A biological marker for low to moderate alcohol use (1-14 drinks/week) during pregnancy would be significant step forward. Preliminary research has shown that fatty acid ethyl esters, long lived non-oxidative metabolites of ethanol, in meconium may serve such a purpose. This proposal seeks to address the hypothesis that fatty acid ethyl esters in meconium are useful biological markers for exposure to low to moderate maternal ethanol use during pregnancy, by achieving the following three specific aims: 1) Determine a denominator to normalize the quantify of fatty acid ethyl esters between subjects. 2) Determining the half of fatty acid ethyl esters in vivo. 3) Using 3 variables, location within the meconium, the quantity and the fatty acid moiety, the quantity and gestational age at exposure can be determined. These studies will use a sheep model of alcohol related neurodevelopmental disorder. These studies will provide preliminary data to study the utility of this biomarker in a human population.

National Institute of Health (NIH)
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Small Research Grants (R03)
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Health Services Research Review Subcommittee (AA)
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Foudin, Laurie L
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Case Western Reserve University
Schools of Medicine
United States
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Gifford, Anne E; Farkas, Kathleen J; Jackson, Leila W et al. (2010) Assessment of benefits of a universal screen for maternal alcohol use during pregnancy. Birth Defects Res A Clin Mol Teratol 88:838-46
Peterson, Jennifer; Kirchner, H Lester; Xue, Wei et al. (2008) Fatty acid ethyl esters in meconium are associated with poorer neurodevelopmental outcomes to two years of age. J Pediatr 152:788-92
Littner, Yoav; Cudd, Timothy A; O'Riordan, Mary A et al. (2008) Elevated fatty acid ethyl esters in meconium of sheep fetuses exposed in utero to ethanol--a new animal model. Pediatr Res 63:164-8
Noland, Julia S; Singer, Lynn T; Short, Elizabeth J et al. (2005) Prenatal drug exposure and selective attention in preschoolers. Neurotoxicol Teratol 27:429-38
Noland, Julia S; Singer, Lynn T; Arendt, Robert E et al. (2003) Executive functioning in preschool-age children prenatally exposed to alcohol, cocaine, and marijuana. Alcohol Clin Exp Res 27:647-56
Bearer, Cynthia F (2003) Meconium as a biological marker of prenatal exposure. Ambul Pediatr 3:40-3
Bearer, C F (2001) Markers to detect drinking during pregnancy. Alcohol Res Health 25:210-8