application): The long range goal of the proposed research is to analyze cytokine regulation of endocytosis and antigen presentation in macrophages in order to maximize the immune response to HIV-1 antigens. This project will test the hypothesis that IL-13 enhances CD4+ T cell responses when used as an adjuvant for HIV-1 gp120 antigens in vivo by increasing macrophage antigen processing within early endosomes, and thereby increasing antigen presentation. To do this, the investigators will immunize mice with bovine RNAase or gp120 SF2 in the presence or absence of IL-13, and test the T cell responses against both RNAase, which has known early and late endosome-derived epitopes, and in parallel, against gp120 epitopes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Small Research Grants (R03)
Project #
5R03AI043206-02
Application #
2887748
Study Section
Acquired Immunodeficiency Syndrome Research Review Committee (AIDS)
Program Officer
Bradac, James A
Project Start
1998-04-15
Project End
2000-03-31
Budget Start
1999-04-01
Budget End
2000-03-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104