Damaged or diseased articular cartilage has limited intrinsic repair capabilities, and the natural history of affected joints is generally poor, leading to progressive arthrosis. The use of fresh human osteochondral allografts to replace articular cartilage defects and resurface areas of osteoarthrosis, and in the reconstruction of acquired osteochondral defects caused by trauma and disease, is an area of growing interest in the arthritis and orthopaedic community. A major obstacle to more wide-spread utilization of allografting is the inherent difficulties in procurement, processing, testing, and delivery of grafts; thus, the number of patients waiting for or eligible to undergo this procedure exceeds the amount of available tissue. This is primarily because the window of permissible storage time for this fresh tissue is narrow; and therefore, presently, these procedures can only be done in specialized centers. In the present study, we propose to assess chondrocyte viability, chondrocyte metabolic activity, cartilage matrix composition, and biomechanical properties within osteochondral plugs stored in different solutions. These solutions will be lactated Ringer's (to serve as a control), allograft storage media, allograft storage media plus fetal bovine serum, and allograft storage media containing defined media supplement insulin, transferrin and selenium (ITS); or vitamin C plus insulin-like growth factor-1. We also propose to investigate the effect of temperature on storage of osteochondral allografts, and the effect of the storage of osteochondral allografts with varying amounts of bone. We hypothesize that the more bone stored with articular cartilage, the greater the adverse effect on cartilage. This study should provide a framework in understanding the optimization of storage conditions for the preservation of osteochondral allografts, as well as a more complete understanding of the scientific basis for osteochondral allograft storage. The data should allow enhanced tissue banking and improve the availability of the limited supply of osteochondral allografts to fulfill the clinical need of these grafts in the treatment of individuals with articular cartilage injury and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Small Research Grants (R03)
Project #
1R03AR049380-01A1
Application #
6710266
Study Section
Special Emphasis Panel (ZAR1-RJB-D (O2))
Program Officer
Panagis, James S
Project Start
2004-09-15
Project End
2007-06-30
Budget Start
2004-09-15
Budget End
2005-06-30
Support Year
1
Fiscal Year
2004
Total Cost
$76,779
Indirect Cost
Name
University of California San Diego
Department
Orthopedics
Type
Schools of Medicine
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Bugbee, William D; Pallante-Kichura, Andrea L; Görtz, Simon et al. (2016) Osteochondral allograft transplantation in cartilage repair: Graft storage paradigm, translational models, and clinical applications. J Orthop Res 34:31-8
Williams, Seth K; Amiel, David; Ball, Scott T et al. (2007) Analysis of cartilage tissue on a cellular level in fresh osteochondral allograft retrievals. Am J Sports Med 35:2022-32
Pennock, Andrew T; Robertson, Catherine M; Wagner, Ferdinand et al. (2006) Does subchondral bone affect the fate of osteochondral allografts during storage? Am J Sports Med 34:586-91
Pennock, Andrew T; Wagner, Ferdinand; Robertson, Catherine M et al. (2006) Prolonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability? J Knee Surg 19:265-72
Allen, R Todd; Robertson, Catherine M; Pennock, Andrew T et al. (2005) Analysis of stored osteochondral allografts at the time of surgical implantation. Am J Sports Med 33:1479-84