Significant advances in understanding amoeboid chemotaxis at the molecular level have been made recently using relatively simple model systems that can be manipulated in culture. One of these, Dictyostelium discoideum, has been particularly valuable in analysis of mechanisms involved in agonist regulated pseudopod extension and chemotaxis. Molecular probes and assays developed with this model have been directly applicable to mammalian cell chemotaxis. We propose to dissect, at the molecular level, the signal transduction pathway which regulates pseudopod formation and chemotaxis in Lewis lung carcinoma cells. In these preliminary studies we will apply the Dictyostelium paradigm for this pathway directly to M27 cells using molecular probes and assays already developed for Dictyostelium. We will test the relationship between receptor mediated actin nucleation activity and cell motility events which are involved in several key steps of metastasis. The roles of several candidate proteins in these steps will be evaluated.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Research Grants (R03)
Project #
1R03CA054932-01
Application #
3423605
Study Section
Special Emphasis Panel (SRC (44))
Project Start
1991-07-15
Project End
1993-06-30
Budget Start
1991-07-15
Budget End
1992-06-30
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461
Condeelis, J; Jones, J; Segall, J E (1992) Chemotaxis of metastatic tumor cells: clues to mechanisms from the Dictyostelium paradigm. Cancer Metastasis Rev 11:55-68