The objectives of the research are: (1) to develop a fundamental understanding of the photophysics for the solid-matrix luminescence (SML) of the highly toxic benzo[a]pyrene (B[a]P)-DNA adducts, dibenzo[a,l]pyrene (DB[a,l]P)-DNA adducts, and both polycyclic aromatic hydrocarbons (PAH) adducts bonded to the same sample of DNA so the basic principles developed can be broadly exploited analytically for a variety of multiple adducted PAH-DNA samples; (2) to elucidate the fundamental photophysical principles of the heavy-atom effect with solid-matrices to characterize B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and the two PAH adducts bonded to the same sample of DNA; (3) to lower the limits of detection for the SML of the B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and both adducts bonded to DNA at the levels found in most human samples; (4) to demonstrate that SML can be used to characterize and detect PAH-DNA adducts in purified blood samples from workers exposed to used motor oil and in purified placental DNA samples. ? ? The expected benefits from the research will be: (1) a fundamental foundation of photophysical knowledge for SML that could be applied broadly to divergent PAH-DNA adducts; (2) highly selective, sensitive, and relatively inexpensive SML methods for the direct characterization of the PAH-DNA adducts individually and in multiple forms; (3) several methods that would be very effective for cancer research related to B[a]P-DNA adducts, DB[a,l]P-DNA adducts, and DNA samples bonded with different types of PAH which are important in assessing the environmental dangers from PAH.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Research Grants (R03)
Project #
5R03CA097723-02
Application #
6658008
Study Section
Special Emphasis Panel (ZCA1-SRRB-3 (M1))
Program Officer
Verma, Mukesh
Project Start
2002-09-13
Project End
2005-08-31
Budget Start
2003-09-01
Budget End
2004-08-31
Support Year
2
Fiscal Year
2003
Total Cost
$46,365
Indirect Cost
Name
University of Wyoming
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
069690956
City
Laramie
State
WY
Country
United States
Zip Code
82071