This project is aimed at determining the expression patterns and the roles of CaMKII in the formation and function of mouse olfactory bulb. The normal expression patterns of CaMKII are essential for understanding this enzyme's functional roles. We will first use the CaMKII specific antibodies to determine where CaMKII is expressed in the mouse olfactory bulb. CaMKII immunoreactive neurons will be further characterized by double labeling with antibodies specific for GABA or glutamate. The ultrastructural location of CaMKII will be determined by electron microscopy, particularly we will examine whether CaMKII is expressed in the reciprocal dendroendritic synapses that are characteristic of the olfactory bulb. In order to understand how CaMKII might function in the olfactory system, we propose to modulate CaMKII activity in the bulb either by using a CaMKII knockout mouse or by expressing active CaMKII or CaMKII inhibitor with Sindbis viruses. In particular, we will investigate whether CaMKII activity regulates the development of granule cells, the most abundant neurons in the olfactory bulb.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Small Research Grants (R03)
Project #
5R03DC004962-02
Application #
6516303
Study Section
Special Emphasis Panel (ZDC1-SRB-O (27))
Program Officer
Davis, Barry
Project Start
2001-05-01
Project End
2004-04-30
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
2
Fiscal Year
2002
Total Cost
$85,250
Indirect Cost
Name
Columbia University (N.Y.)
Department
Biology
Type
Other Domestic Higher Education
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027
Zou, Dong-Jing; Greer, Charles A; Firestein, Stuart (2002) Expression pattern of alpha CaMKII in the mouse main olfactory bulb. J Comp Neurol 443:226-36