Our goals are to understand whether the intrinsic ion-channel properties of auditory neurons shape their function. Specifically, we want to understand how some groups of Type I auditory neurons encode low-intensity sounds whereas others encode high-intensity sounds. The mechanisms shaping these response features are unknown but are likely to rely on both pre- and post-synaptic specializations. Recordings from the isolated somata of auditory neurons suggest that diversity in ion channel properties may influence their response, but a clear correlation has not been demonstrated. To make direct correlations, we propose to use semi-intact in vitro preparations of rat cochleae that preserve the anatomical and functional connections between the spiral ganglion and organ of Corti. The approach brings together whole-cell patch-clamp recordings, neuronal labeling, morphometric analysis, and computational modeling to explore how the biophysical properties intrinsic to primary auditory afferent neurons shape their physiological responses. Furthermore, because our experiments are from animals at ages before and after the onset of hearing, we can characterize the time course over which Type I auditory neurons'mature biophysically and morphologically into distinct functional groups.

Public Health Relevance

The neurons of the auditory nerve are the primary conduit for information from the ear to the brain. Some of these neurons convey information about soft sounds while others convey loud sounds. Selective damage to one population may be responsible for some forms of age related hearing loss. Our work seeks to understand whether the membrane proteins of these different groups of neurons differ, and whether such difference underlies their differential sensitivity to damage.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Small Research Grants (R03)
Project #
1R03DC012652-01A1
Application #
8502163
Study Section
Communication Disorders Review Committee (CDRC)
Program Officer
Cyr, Janet
Project Start
2013-03-01
Project End
2013-09-30
Budget Start
2013-03-01
Budget End
2013-09-30
Support Year
1
Fiscal Year
2013
Total Cost
$29,906
Indirect Cost
$13,152
Name
House Research Institute
Department
Type
DUNS #
062076989
City
Los Angeles
State
CA
Country
United States
Zip Code
90057