This is the second revision of a small grant application which proposes to study the relationship between plasminogen activator (PA) and plasminogen activator inhibitor (PAI) activities in gingival tissue obtained from diabetic or non-diabetic rats. Gingival collagenase activity has previously been shown to be increased in periodontal disease and in diabetes. Although the in vivo activators of latent collagenase are unknown, one pathway may involve the action of plasmin, which is generated from plasminogen by the action of PAs, especially tissue plasminogen activator (t-PA). Plasminogen activator activity is in turn down regulated by PA inhibitors (PAI). The balance between PA and PAI may regulate collagenolytic activity.
The specific aims are to determine, in diabetic and non-diabetic rats: 1) PA and PAI activities, 2) gingival IL-1 and TNFa activities, and 3) the severity of inflammation, alveolar bone and attachment loss. The long-term goal of these studies is to elucidate the mechanisms of connective tissue breakdown in diabetes-associated periodontal disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Small Research Grants (R03)
Project #
1R03DE010657-01A2
Application #
2131553
Study Section
NIDCR Special Grants Review Committee (DSR)
Project Start
1994-09-01
Project End
1996-08-31
Budget Start
1994-09-01
Budget End
1995-08-31
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Medicine & Dentistry of NJ
Department
Dentistry
Type
Schools of Dentistry
DUNS #
605799469
City
Newark
State
NJ
Country
United States
Zip Code
07107
Chang, K; Rani, A S; Chang, K et al. (1996) Plasminogen activator activity is decreased in rat gingiva during diabetes. J Periodontol 67:743-47
Chang, K M; Lehrhaupt, N; Lin, L M et al. (1996) Epidermal growth factor in gingival crevicular fluid and its binding capacity in inflamed and non-inflamed human gingiva. Arch Oral Biol 41:719-24