This revised proposal is designed to characterize the regulation of expression of the dentin matrix protein - 1 (DMP-1) gene. DMP-1 is a secreted protein found predominately in dentin, but DMP-1 mRNA is also detected by in situ hybridization in osteoblasts. The hypothesis to be tested is that tissue specificity is due to interactions between transcription factors and gene DNA sequences in the DMP-1 promoter and upstream regions.
The specific aims are to (1) characterize and compare DNA sequences for the human and murine DMP-1 genes; (2) determine upstream regions that confer tissue-specific expression using gene transfer of expression vectors into cultured odontoblasts and other cell types; and (3) to determine the importance of methylation in tissue specific expression.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Small Research Grants (R03)
Project #
7R03DE012057-03
Application #
2884864
Study Section
Special Emphasis Panel (ZDE1-GH (06))
Project Start
1997-05-01
Project End
2000-04-30
Budget Start
1998-11-01
Budget End
2000-04-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Missouri Kansas City
Department
Dentistry
Type
Schools of Dentistry
DUNS #
800772162
City
Kansas City
State
MO
Country
United States
Zip Code
64110
Fen, Jian Q; Zhang, Jianghong; Dallas, Sarah L et al. (2002) Dentin matrix protein 1, a target molecule for Cbfa1 in bone, is a unique bone marker gene. J Bone Miner Res 17:1822-31