This research aims to study chemotaxis of Treponema denticola. T. denticola, a motile gram negative oral spirochete, was found to be associated with periodontal diseases. Previous studies have recognized the role motility plays in allowing the bacterium to penetrate periodontal tissues and migrate to sites favorable to bacteria] proliferation. This grant application proposes that the motility of T. denticola is controlled by a chemotaxis system and aim to study the chemotaxis genes and chemotactic behavior of T. denticola. Summarized here are the proposed experiments: 1) chemotaxis genes will be identified, cloned and sequenced using a newly isolated cheA PCR fragment as the probe; 2) chemotaxis mutants will be constructed based on insertional gene disruption and homologous recombination; and 3) the chemotaxis mutants will be characterized using the chemotaxis assays developed in the laboratory. The knowledge learned from these studies could give insights into the molecular mechanisms of pathogenesis of T. denticola and other spirochete-related diseases.
|Lux, Renate; Sim, Jee-Hyun; Tsai, Jon P et al. (2002) Construction and characterization of a cheA mutant of Treponema denticola. J Bacteriol 184:3130-4|
|Gu, Fang; Lux, Renate; Anderson, Maxwell H et al. (2002) Analyses of Streptococcus mutans in saliva with species-specific monoclonal antibodies. Hybrid Hybridomics 21:225-32|
|Gu, Fang; Ma, Xiaoyuan; Lux, Renate et al. (2002) Production and characterization of species-specific monoclonal antibodies against Actinomyces naeslundii and Lactobacillus casei. Hybrid Hybridomics 21:469-78|
|Lux, R; Miller, J N; Park, N H et al. (2001) Motility and chemotaxis in tissue penetration of oral epithelial cell layers by Treponema denticola. Infect Immun 69:6276-83|
|Tsai, J P; Shi, W (2000) Analysis of gene expression in Treponema denticola with differential display polymerase chain reaction. Oral Microbiol Immunol 15:305-8|
|Lux, R; Moter, A; Shi, W (2000) Chemotaxis in pathogenic spirochetes: directed movement toward targeting tissues? J Mol Microbiol Biotechnol 2:355-64|