The goal of this application is to determine the post-transcriptional mechanisms of gene expression control of the MeCP2 gene. The principal investigator will delineate alternative polyadenylation of MeCP2, the process that determines the size of the 3' UTR in a tissue-and developmentally-specific fashion. She will also investigate the role that mRNA stability may play in MeCP2 expression regulation. These studies will be performed in vivo in a variety of cell lines with special attention to neural cells. The investigator will then turn to established in vitro systems using these cell lines in order to investigate mechanisms of regulation by alternative polyadenylation and differential RNA stability. These studies represent an under-explored area of research on MeCP2 gene expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome. ? ? ?

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Small Research Grants (R03)
Project #
1R03HD054559-01
Application #
7184526
Study Section
Pediatrics Subcommittee (CHHD)
Program Officer
Oster-Granite, Mary Lou
Project Start
2007-03-15
Project End
2009-02-28
Budget Start
2007-03-15
Budget End
2008-02-29
Support Year
1
Fiscal Year
2007
Total Cost
$77,916
Indirect Cost
Name
University of Medicine & Dentistry of NJ
Department
Biochemistry
Type
Schools of Medicine
DUNS #
623946217
City
Newark
State
NJ
Country
United States
Zip Code
07107
Lutz, Carol S; Moreira, Alexandra (2011) Alternative mRNA polyadenylation in eukaryotes: an effective regulator of gene expression. Wiley Interdiscip Rev RNA 2:23-31
Lutz, Carol S; Moreira, Alexandra (2011) Alternative mRNA polyadenylation in eukaryotes: an effective regulator of gene expression. Wiley Interdiscip Rev RNA 2:22-31