The proposed research addresses the ability of brain blood vessels to adapt their responsiveness, local blood flow and architecture to alterations in the cerebral cortex during cortical development, in response to neural activity, and after sensory deafferentation or deprivation. We use the well established model system, the whisker-- barrel cortex of the rat. This collaboration combines the complementary talents and resources of cerebrovascular laboratories in the Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences, St. Petersburg, Russian Republic, USSR, and in Washington University, St. Louis, Missouri, USA. This proposal will expand and enhance two research projects funded by NIH: (a) ROlHL41075 """"""""Models for brain angiogenesis"""""""", and (b) R01 NS28781 """"""""Imaging brain blood vessels during cortical activity"""""""". It will facilitate collaborative and correlative research on local cerebral blood flow, P02, evoked activity, angiogenesis, and vascular remodeling. Methods for videomicroscopy in vivo, fluorescent tracers, and histology of the barrel cortex of rodents are well established in St. Louis. The laboratory in St. Petersburg provides expertise in biophysical measurements of local cerebral blood flow (ICBF), extracellular fluid space (ECS), and tissue P02, using the same barrel cortex model. The expected results will include the correlation of physiological and videomicroscopic parameters in normal barrel cortex of rats and remodeling of cerebral blood vessels during development and after selective sensory deafferentation.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW000053-02
Application #
3432469
Study Section
Special Emphasis Panel (SRC)
Project Start
1992-02-20
Project End
1995-02-19
Budget Start
1993-02-20
Budget End
1994-02-19
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Moskalenko YuE; Rovainen, C; Woolsey, T A et al. (1996) Comparison of measurements of local brain blood flow by hydrogen clearance with the inhalation of hydrogen and its electrochemical generation in brain tissue. Neurosci Behav Physiol 26:245-50
Vainshtein, G B; Zhuravin, I A; Rovainen, C et al. (1996) [Cerebral blood flow and cerebrovascular reactivity in the early postnatal ontogeny of rats] Zh Evol Biokhim Fiziol 32:160-6
Moskalenko, Iu E; Rovainen, C M; Woolsey, T A et al. (1994) [Combinations of methods for the monitoring of the cerebral microcirculation] Fiziol Zh Im I M Sechenova 80:144-53