The long-term objective of this collaborative project is to broaden current studies of the persistence of the AIDS virus and other lentiviruses to encompass retroviruses in general, utilizing a biological model developed by Dr. Svoboda. Conventional in situ and other hybridization techniques and new amplification methods will be employed in defining the cellular and anatomic sites of persistence and the relationship of viral gene expression to persistence. Retroviral genomes will also be characterized at the nucleotide sequence level to assess the role of mutation and diversity in establishing and maintaining infection.
| Svoboda, J; Hejnar, J; Geryk, J et al. (2000) Retroviruses in foreign species and the problem of provirus silencing. Gene 261:181-8 |
| Hejnar, J; Plachy, J; Geryk, J et al. (1999) Inhibition of the rous sarcoma virus long terminal repeat-driven transcription by in vitro methylation: different sensitivity in permissive chicken cells versus mammalian cells. Virology 255:171-81 |
| Machon, O; Strmen, V; Hejnar, J et al. (1998) Sp1 binding sites inserted into the rous sarcoma virus long terminal repeat enhance LTR-driven gene expression. Gene 208:73-82 |
| Geryk, J; Machon, O; Hak, R et al. (1996) Frequent detection of reviraemia in ducks persistently infected with avian leukosis retroviruses. Folia Biol (Praha) 42:245-55 |
| Machon, O; Hejnar, J; Hajkova, P et al. (1996) The LTR, v-src, LTR provirus in H-19 hamster tumor cell line is integrated adjacent to the negative regulatory region. Gene 174:9-17 |
