Abstract

The proposed research involves investigating the molecular mechanisms which control the selective degradation of peroxisomes in Pichia pastoris. It is proposed to isolate mutants that are defective in the degradation of methanol degrading peroxisomes and to characterize these biochemically and cytologically. The peroxisome degrading genes will be isolated by functional complementation, sequenced and the amino acid sequence deduced. Mutant and wild type sequences will be compared to provide insight concerning function.
The second aim of the proposed research is to isolate peroxisome biogenesis defective mutants via the development of new approaches for positive selection. The physiological characteristics of these mutants will be studied.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW000547-03
Application #
2546693
Study Section
International and Cooperative Projects 1 Study Section (ICP)
Program Officer
Michels, Kathleen M
Project Start
1995-09-30
Project End
1998-09-29
Budget Start
1997-09-30
Budget End
1998-09-29
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Oregon Health and Science University
Department
Engineering (All Types)
Type
Other Domestic Higher Education
DUNS #
009584210
City
Portland
State
OR
Country
United States
Zip Code
97239

  Publications

Stasyk, Oleh V; Nazarko, Taras Y; Sibirny, Andriy A (2008) Methods of plate pexophagy monitoring and positive selection for ATG gene cloning in yeasts. Methods Enzymol 451:229-39
Stasyk, Oleh V; Stasyk, Olena G; Mathewson, Richard D et al. (2006) Atg28, a novel coiled-coil protein involved in autophagic degradation of peroxisomes in the methylotrophic yeast Pichia pastoris. Autophagy 2:30-8