This application aims to study whether NO induced by cytokines can affect HIV-1 provirus synthesis. To accomplish this, the specific aims are to construct retroviral vectors which have inducible NO synthetase under tat regulation.
In Aim 2, the application will analyze the effect of NO synthetase over-expression on HIV replication.
In Aim 3, the application will examine the effect of NO on integrated HIV-1 provirus and HIV transcription in chronically-infected lines.
Aim 4 is to determine the NO effect on the expression of IFN-a resistant HIV-1 isolates. These studies might lead to new therapies for HIV replication.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW000808-02
Application #
2655613
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1997-02-01
Project End
2000-01-31
Budget Start
1998-02-01
Budget End
1999-01-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218