The overall aim of the U.S. Principal Investigator's NIH Grant AI 37139 is to investigate the role of lipoarabinomanan (LAM) in the virulence and pathogenesis of tuberculosis and leprosy. Identification of truncated structural variants of LAM in ethambutol drug-resistant strains of mycobacteria has provided the PI with model compounds for both structural and functional studies aimed at defining the relevance of LAM in host-mycobacterial interactions. The present AIDS-FIRCA proposal is to investigate the structural features of LAM from clinical and laboratory strains of M. avium complex (MAC) that differ in colony morphology and drug resistance. Specifically, the proposal will address relationship of colony morphotypes, virulence and drug reasistance by defining the chemical nature of LAM from MAC, by examinig the effect of ethambutol on the arabinan biosynthesis in the cell wall of MAC, and by studying the biochemical markers that distinguish virulent and avirulent isolates.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW000943-02
Application #
2751059
Study Section
AIDS and Related Research Study Section 5 (ARRE)
Project Start
1997-09-30
Project End
2000-07-31
Budget Start
1998-08-01
Budget End
1999-07-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Microbiology/Immun/Virology
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523
Hale-Donze, Hollie; Greenwell-Wild, Teresa; Mizel, Diane et al. (2002) Mycobacterium avium complex promotes recruitment of monocyte hosts for HIV-1 and bacteria. J Immunol 169:3854-62