The overall objective of the proposed collaborative study between the University of Pittsburgh, USA and the Chittaranjan National Cancer Institute at Calcutta, India is to determine the effect of non-B HIV-1 strains presumably present in Calcutta on CD+ T cell mediated suppression of HIV-1. This study is an extension of a parent grant of the principal investigator to determine the mechanism of CD8+ T cell mediated suppression of HIV-1.
Specific aims of the study are to determine the sero prevalence of HIV-1 and to identify HIV-1 infected subjects among commercial sex workers, subjects attending STD Clinic and paid blood donors in Calcutta. 2. To determine the genotypes of HIV-1 present in PBMC from infected subjects from Calcutta by HMA and DNA sequence analysis of the V3-V5 region of the envelope and 3. To compare the level of CD8+ T cell mediated suppressive activity against HIV-1 strains isolated from Calcutta and the US using the investigator's developed semi-quantitative assay for suppression. The proposed study of evaluating antiviral activity of CD8+ T cells against HIV-1 strains of various clades will be extremely important for determining the mechanism of CD8+ T cell mediated suppression of HIV-1. In addition, the information obtained from the proposed study would be useful in the development of an HIV-1 vaccine which ideally would be expected to induce CD8+ T cell mediated suppression against HIV-1 of all clades.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
5R03TW000971-02
Application #
6078388
Study Section
AIDS and Related Research Study Section 1 (ARRA)
Project Start
1998-09-30
Project End
2001-09-29
Budget Start
1999-09-30
Budget End
2000-09-29
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Internal Medicine/Medicine
Type
Schools of Public Health
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Gupta, Phalguni; Kingsley, Lawrence; Sheppard, Haynes W et al. (2003) High incidence and prevalence of HIV-1 infection in high risk population in Calcutta, India. Int J STD AIDS 14:463-8