The parent project for this FIRCA application is a project within a larger Program Project grant whose PI is Dr. Bruce Weir. Drs. Mackay and Charles Langley are key investigators on Project 6, which is entitled Drosophila Quantitative Genetics.
The aims of the parent project are to use QTL mapping methods to characterize the genetic basis of and forces that influence genetic variation of a quantitative trait in Drosophila, bristle number. The parent grant will utilize very creative approaches to clarifying the genetic factors. The goals of the FIRCA grant are to employ a subset of these techniques in order to develop a set of 500 recombinant inbred (RI) strains from natural populations of Drosophila in order to characterize the genetic loci that may influence longevity. Using QTL methodology, the strains will be backcrossed to the parental lines, and a 2cM dense marker map (using insertion sites of roo transposable elements)will be employed. Evidence for QTL to longevity will be scored by the difference in mean trait value between marker classes. The investigators then will extend the QTL analysis to examine gene x environment interaction by changing the environment of the flies from optimal conditions (25oC) to stressful (14oC and 29oC) and measure changes in longevity.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
Small Research Grants (R03)
Project #
1R03TW000997-01
Application #
2695514
Study Section
International and Cooperative Projects 1 Study Section (ICP)
Project Start
1998-09-30
Project End
2001-09-29
Budget Start
1998-09-30
Budget End
1999-09-29
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
North Carolina State University Raleigh
Department
Genetics
Type
Schools of Earth Sciences/Natur
DUNS #
City
Raleigh
State
NC
Country
United States
Zip Code
27695