This research will be done primarily in Russia as an extension of NIH grant no.GM21457. The research will focus on the interaction of water-soluble polymers with vesicular biomembranes, a topic of considerable biomedical relevance. The project will combine the expertise of the Yaroslavov group in Moscow (photon correlation spectroscopy, laser microelectrophoresis, conductometry etc.) with that of the Menger group (organic synthesis, light and electron microscopy, colloid chemistry). An active collaboration, which has already resulted in several joint publications, has existed for four years, but the time has come when direct support is needed to expand the effort and have the collaboration reach its full potential. The main thrust will be to interact synthetic polymers, with varying content of ionic groups and hydrophobic chains, with giant vesicles that can be observed under the light microscope. Giant vesicles are composed of single phospholipid bilayers and have been shown by the Menger group to undergo """"""""cytomimetic"""""""" events including fusion, fission, budding, endocytosis, and aggregation. The effect of polymer structure, and membrane composition, upon these cell-like morphological changes has never been elucidated. Work with giant vesicles will be coupled to work with more conventional large unilamellar vesicles. By using a combination of giant and large vesicles, changes in membrane morphology will be related to molecular structure of interacting polymers as well as composition and properties of polymer-vesicle complexes. The hope, generally speaking, is to obtain a wealth of information on membrane behavior in response to macromolecular binding using defined, structurally controllable systems and both visual and instrumental analyses.
| Yaroslavov, Alexander A; Sybachin, Andrey V; Kesselman, Ellina et al. (2011) Liposome fusion rates depend upon the conformation of polycation catalysts. J Am Chem Soc 133:2881-3 |
| Yaroslavov, Alexander A; Sybachin, Andrei V; Schrinner, Marc et al. (2010) Liposomes remain intact when complexed with polycationic brushes. J Am Chem Soc 132:5948-9 |
| Yaroslavov, Alexander A; Sitnikova, Tatiana A; Rakhnyanskaya, Anna A et al. (2009) Biomembrane sensitivity to structural changes in bound polymers. J Am Chem Soc 131:1666-7 |
| Davydov, Dmitry A; Yaroslavova, Ekaterina G; Rakhnyanskaya, Anna A et al. (2009) Polymer migration among phospholipid liposomes. Langmuir 25:13528-33 |
| Yaroslavov, Alexander A; Rakhnyanskaya, Anna A; Yaroslavova, Ekaterina G et al. (2008) Polyelectrolyte-coated liposomes: stabilization of the interfacial complexes. Adv Colloid Interface Sci 142:43-52 |
| Sybachin, A V; Efimova, A A; Litmanovich, E A et al. (2007) Complexation of polycations to anionic liposomes: composition and structure of the interfacial complexes. Langmuir 23:10034-9 |
| Yaroslavov, A A; Sitnikova, T A; Rakhnyanskaya, A A et al. (2007) Contrasting behavior of zwitterionic and cationic polymers bound to anionic liposomes. Langmuir 23:7539-44 |
| Yaroslavov, Alexander A; Melik-Nubarov, Nikolay S; Menger, Fredric M (2006) Polymer-induced flip-flop in biomembranes. Acc Chem Res 39:702-10 |
| Kitaeva, M V; Melik-Nubarov, N S; Menger, F M et al. (2004) Doxorubicin-poly(acrylic acid) complexes: interaction with liposomes. Langmuir 20:6575-9 |
| Menger, Fredric M; Seredyuk, Victor A; Yaroslavov, Alexander A (2002) Adhesive and anti-adhesive agents in giant vesicles. Angew Chem Int Ed Engl 41:1350-2 |
