This research will be done at the University of Rochester and the Polish Academy of Sciences Institute of Bioorganic Chemistry in Poznan, Poland. The work will be in collaboration with Prof. Douglas H. Turner at the University of Rochester as an extension of NIH Grant No. RO1GM22939, 8-15-2008 to 7-31-2012, and will include additional collaborators at the University of Rochester (D.H. Mathews, S.D. Kennedy, and B. Kim) and at the State University of New York at Buffalo (M.D. Disney). The research will be done by Dr. Elzbieta Kierzek, who is the equivalent of an Assistant Professor at the Institute of Bioorganic Chemistry in Poznan. The goals of the work are to improve methods for predicting and determining the RNA secondary structures of influenza virus and to discover compounds that can lead to therapeutics for influenza RNA.

Public Health Relevance

Viruses with RNA genomes are responsible for many diseases, including influenza. This project studies methods to determine rapidly the structures of influenza RNA and to discover compounds capable of therapeutically targeting the RNA.

National Institute of Health (NIH)
Fogarty International Center (FIC)
Small Research Grants (R03)
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International and Cooperative Projects - 1 Study Section (ICP1)
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Sina, Barbara J
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University of Rochester
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Lenartowicz, Elzbieta; Nogales, Aitor; Kierzek, Elzbieta et al. (2016) Antisense Oligonucleotides Targeting Influenza A Segment 8 Genomic RNA Inhibit Viral Replication. Nucleic Acid Ther 26:277-285
Lenartowicz, Elzbieta; Kesy, Julita; Ruszkowska, Agnieszka et al. (2016) Self-Folding of Naked Segment 8 Genomic RNA of Influenza A Virus. PLoS One 11:e0148281
Lisowiec, Jolanta; Magner, Dorota; Kierzek, Elzbieta et al. (2015) Structural determinants for alternative splicing regulation of the MAPT pre-mRNA. RNA Biol 12:330-42
Soszynska-Jozwiak, Marta; Michalak, Paula; Moss, Walter N et al. (2015) A Conserved Secondary Structural Element in the Coding Region of the Influenza A Virus Nucleoprotein (NP) mRNA Is Important for the Regulation of Viral Proliferation. PLoS One 10:e0141132
Kierzek, Ryszard; Turner, Douglas H; Kierzek, Elzbieta (2015) Microarrays for identifying binding sites and probing structure of RNAs. Nucleic Acids Res 43:1-12
Magner, Dorota; Biala, Ewa; Lisowiec-Wachnicka, Jolanta et al. (2015) A Tandem Oligonucleotide Approach for SNP-Selective RNA Degradation Using Modified Antisense Oligonucleotides. PLoS One 10:e0142139
Jiang, Tian; Kennedy, Scott D; Moss, Walter N et al. (2014) Secondary structure of a conserved domain in an intron of influenza A M1 mRNA. Biochemistry 53:5236-48
Kierzek, Elzbieta; Malgowska, Magdalena; Lisowiec, Jolanta et al. (2014) The contribution of pseudouridine to stabilities and structure of RNAs. Nucleic Acids Res 42:3492-501
Zhou, Yuan; Kierzek, Elzbieta; Loo, Zi Ping et al. (2013) Recognition of RNA duplexes by chemically modified triplex-forming oligonucleotides. Nucleic Acids Res 41:6664-73
Priore, Salvatore F; Kierzek, Elzbieta; Kierzek, Ryszard et al. (2013) Secondary structure of a conserved domain in the intron of influenza A NS1 mRNA. PLoS One 8:e70615

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