EXCEED THE SPACE PROVIDED. Our long-term goal is to understand the health-related effects of consuming polyphenols found in plant- based foods and beverages. Eating a diet rich in fruits, vegetables, and plant-derived beverages may reduce the risk of diseases that have been linked to oxidative stress, including inflammatory bowel disease, cardiovascular disease and cancer. Plants are a source of nutritive antioxidarits (vitamins) but may also be a source of non-nutritive antioxidants. Polyphenols are the most common group of plant secondary products, and are effective antioxidants in vitro. We believe that the specific class of polyphenols known as the polymeric polyphenols have unique attributes not shared by other non-nutritive antioxidants. Polymeric polyphenols bind proteins with high affinity, so that polyphenol-protein complexes are the major species in the gastrointestinal tract. We speculate that binding to protein alters the properties?such as lifetime and antioxidant activities?of polymeric polyphenols, making them uniquely suited to alleviating inflammatory bowel disease. We hypothesize that polymeric polyphenol- protein adducts provide a pool of persistant, high capacity activity that is localized to the Gl tract and is thus able to alleviate oxidative damage and inflammation directly at the site of inflammatory bowel disease. There are thousands of plant polyphenols, in several distinct structural classes. Conducting in vivo tests of our hypothesis would be impossible because it is not clear which compounds might be most bioactive. We propose in vitro studies to examine the persistence of ten different polyphenols and their protein adducts under gastrointestinal conditions. We will then examine the antioxidant and anti-inflammatory activities of these polyphenols and their protein adducts. This should allow us to identify types of dietary polyphenols that are most likely to alleviate inflammatory bowel disease in vivo, and position us to continue with testing in animals in a future proposal. PERFORMANCE SITE ========================================Section End===========================================

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
3R15DK069285-01A1S1
Application #
8011852
Study Section
Xenobiotic and Nutrient Disposition and Action Study Section (XNDA)
Program Officer
May, Michael K
Project Start
2010-02-01
Project End
2010-08-31
Budget Start
2010-02-01
Budget End
2010-08-31
Support Year
1
Fiscal Year
2010
Total Cost
$14,681
Indirect Cost
Name
Miami University Oxford
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
041065129
City
Oxford
State
OH
Country
United States
Zip Code
45056
Li, M; Hagerman, A E (2015) Effect of (-)-epigallocatechin-3-gallate on glucose-induced human serum albumin glycation. Free Radic Res 49:946-53
Li, Min; Hagerman, Ann E (2014) Role of the flavan-3-ol and galloyl moieties in the interaction of (-)-epigallocatechin gallate with serum albumin. J Agric Food Chem 62:3768-75
Krook, Melanie A; Hagerman, Ann E (2012) Stability of Polyphenols Epigallocatechin Gallate and Pentagalloyl Glucose in a Simulated Digestive System. Food Res Int 49:112-116
Trombley, John D; Loegel, Thomas N; Danielson, Neil D et al. (2011) Capillary electrophoresis methods for the determination of covalent polyphenol-protein complexes. Anal Bioanal Chem 401:1523-9
Wisman, Kimberly N; Perkins, Akeysha A; Jeffers, Melanie D et al. (2008) Accurate assessment of the bioactivities of redox-active polyphenolics in cell culture. J Agric Food Chem 56:7831-7
Hofmann, Thomas; Glabasnia, Arne; Schwarz, Bernd et al. (2006) Protein binding and astringent taste of a polymeric procyanidin, 1,2,3,4,6-penta-O-galloyl-beta-D-glucopyranose, castalagin, and grandinin. J Agric Food Chem 54:9503-9