Abstract

This is an AREA grant application that combines both solid-state nuclear magnetic resonance (NMR) spectroscopy and electron paramagnetic resonance (EPR) spectroscopy to investigate the structural and dynamic properties of the membrane-bound protein phospholamban, shown to regulate Ca-ATPase activity in the sarcoplasmic reticulum of cardiac muscle cells. In particular, the orientation of phospholamban will be determined with respect to the lipid bilayer, complementary dynamic information will also be obtained in two different time scale regimes, and new methods for orienting lipid bicelle systems at lower magnetic fields for EPR spectroscopic studies are proposed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15GM060259-01
Application #
6028233
Study Section
Biophysical Chemistry Study Section (BBCB)
Program Officer
Chin, Jean
Project Start
2000-03-01
Project End
2004-02-29
Budget Start
2000-03-01
Budget End
2004-02-29
Support Year
1
Fiscal Year
2000
Total Cost
$105,000
Indirect Cost

  Institution

Name
Miami University Oxford
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
041065129
City
Oxford
State
OH
Country
United States
Zip Code
45056

  Publications

Ghimire, Harishchandra; Hustedt, Eric J; Sahu, Indra D et al. (2012) Distance measurements on a dual-labeled TOAC AChR M2? peptide in mechanically aligned DMPC bilayers via dipolar broadening CW-EPR spectroscopy. J Phys Chem B 116:3866-73
Ghimire, Harishchandra; Abu-Baker, Shadi; Sahu, Indra D et al. (2012) Probing the helical tilt and dynamic properties of membrane-bound phospholamban in magnetically aligned bicelles using electron paramagnetic resonance spectroscopy. Biochim Biophys Acta 1818:645-50
Nusair, Nisreen A; Mayo, Daniel J; Dorozenski, Tia D et al. (2012) Time-resolved EPR immersion depth studies of a transmembrane peptide incorporated into bicelles. Biochim Biophys Acta 1818:821-8
Mayo, Daniel; Zhou, Andy; Sahu, Indra et al. (2011) Probing the structure of membrane proteins with electron spin echo envelope modulation spectroscopy. Protein Sci 20:1100-4
Yu, Xueting; Chu, Shidong; Hagerman, Ann E et al. (2011) Probing the interaction of polyphenols with lipid bilayers by solid-state NMR spectroscopy. J Agric Food Chem 59:6783-9
Newstadt, Justin P; Mayo, Daniel J; Inbaraj, Johnson J et al. (2009) Determining the helical tilt of membrane peptides using electron paramagnetic resonance spectroscopy. J Magn Reson 198:1-7
Ghimire, Harishchandra; Inbaraj, Johnson J; Lorigan, Gary A (2009) A comparative study of the effect of cholesterol on bicelle model membranes using X-band and Q-band EPR spectroscopy. Chem Phys Lipids 160:98-104
Chu, Shidong; Hawes, John W; Lorigan, Gary A (2009) Solid-state NMR spectroscopic studies on the interaction of sorbic acid with phospholipid membranes at different pH levels. Magn Reson Chem 47:651-7
Ghimire, Harishchandra; McCarrick, Robert M; Budil, David E et al. (2009) Significantly improved sensitivity of Q-band PELDOR/DEER experiments relative to X-band is observed in measuring the intercoil distance of a leucine zipper motif peptide (GCN4-LZ). Biochemistry 48:5782-4
Gullà, Stefano V; Sharma, Gaurav; Borbat, Peter et al. (2009) Molecular-scale force measurement in a coiled-coil peptide dimer by electron spin resonance. J Am Chem Soc 131:5374-5

Showing the most recent 10 out of 35 publications