Alcohol dependence and relapse in abstinent alcoholics are major health problems in the United States and neurochemical pathways that modulate these disorders are currently under investigation. However, the central mechanisms involved in the regulation of non-dependent binge alcohol drinking have received far less attention. Of great concern, binge drinking during adolescent years can significantly increases the risk of developing ethanol dependence later in life. Thus, it is of paramount importance to identify the central mechanisms that modulate the effects of adolescent binge drinking on subsequent excessive ethanol intake in adulthood. The role of epigenetic factors (i.e. mechanisms that influence changes of gene expression independent of changes to the underlying DNA sequence) in ethanol consumption is a new approach to understand the mechanisms that modulate excessive ethanol drinking. Brain chromatin remodeling regulates gene expression via enzymatic restructuring of histone proteins and DNA without altering the primary genetic sequence. Recent evidence has suggested that epigenetic mechanisms contribute to ethanol-induced transcriptional changes. However, the role of histone methylation in binge-like ethanol drinking has not been addressed. An interesting possibility is that adolescent ethanol exposure facilitates changes in histone methylation in critical brain regions, and this increases the risk of excessive ethanol drinking in adult years. We have found that binge-like ethanol drinking in adult mice is associated with decreased H3 lysine 9 dimethylation (H3K9me2) in the nucleus accumbens (NAc). Therefore, the specific aims proposed below will test the guiding hypotheses that (A) H3K9me2 activity in the NAc modulates excessive ethanol drinking in adult mice, and that (B) adolescent binge-like ethanol drinking promotes robust and long- term reductions of histone dimethylation in brain regions implicated in ethanol intake, and these changes contribute to increased ethanol drinking in adulthood.
Aim 1 will test the hypothesis that deletion of H3K9me2 in the NAc will augment binge-like ethanol drinking, and that overexpression of G9a (and H3K9me2 activity) in the NAc will protect against binge-like ethanol drinking, in adult mice.
Aim 2 A will determine if a history of binge-like ethanol drinking will blunt H3K9me2 and enzymes responsible for histone dimethylation in brain regions implicated in binge-like drinking, and if this effect will be greater in adolescent than adult mice and persist into adulthood.
Aim 2 B will test the hypothesis that pharmacological inhibition of G9a in adolescent mice will increased voluntary ethanol drinking in adulthood, and determine if overexpression of G9a protects against increased voluntary ethanol intake in adulthood stemming from adolescent binge drinking. These studies will provide a set of data and proof of concept for powerful and innovative tools that will be the foundation for a future R01 application that will continue research on the role of epigenetic mechanism in modulating the neurobiological responses associated with adolescent ethanol drinking.

Public Health Relevance

Adolescent binge drinking is a growing problem with numerous health consequences, including an increased risk of developing alcohol dependence later in life. Thus, it is of great importance to identify the neurobiological mechanisms that underlie the effects of adolescent ethanol drinking on subsequent excessive ethanol consumption in adulthood. This highly innovative proposal will allow us to test the hypothesis that epigenetic mechanisms (specifically histone dimethylation) play key roles, and at the same time provide insight into new tools for studying the role of H3K9me2 in adolescent binge-like ethanol drinking.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21AA022044-02
Application #
8843315
Study Section
Special Emphasis Panel (ZAA1)
Program Officer
Reilly, Matthew
Project Start
2014-05-01
Project End
2017-04-30
Budget Start
2015-05-01
Budget End
2017-04-30
Support Year
2
Fiscal Year
2015
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Psychology
Type
Schools of Arts and Sciences
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
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Sprow, Gretchen M; Rinker, Jennifer A; Lowery-Gointa, Emily G et al. (2016) Lateral hypothalamic melanocortin receptor signaling modulates binge-like ethanol drinking in C57BL/6J mice. Addict Biol 21:835-46
Navarro, Montserrat; Olney, Jeffrey J; Burnham, Nathan W et al. (2016) Lateral Hypothalamus GABAergic Neurons Modulate Consummatory Behaviors Regardless of the Caloric Content or Biological Relevance of the Consumed Stimuli. Neuropsychopharmacology 41:1505-12
Navarro, Montserrat; Carvajal, Francisca; Lerma-Cabrera, Jose Manuel et al. (2015) Evidence that Melanocortin Receptor Agonist Melanotan-II Synergistically Augments the Ability of Naltrexone to Blunt Binge-Like Ethanol Intake in Male C57BL/6J Mice. Alcohol Clin Exp Res 39:1425-33