HIV remains the most serious infectious disease challenge to public health. As a result of inadequate access to HIV prevention and treatment, everyday, more than 6800 people contract HIV and more than 5700 people die from AIDS, globally. In 2007, worldwide, 33.2 million people were living with HIV infection, 2.5 million people were newly infected and 2.1 million died from AIDS mostly due to lack of available monitoring technologies at resource limited settings. Our objective is to develop a rapid, accurate and low-cost HIV monitoring device using a novel CD4+ T lymphocyte purification and counting technology at resource limited settings. We have been working on microfluidic CD4+ T cell counting microchips and also tested them with HIV patient samples. Here, we will leverage from this expertise to create an on-chip CD4+ T cell purification technology that can eliminate CD4+ monocytes to achieve +10% clinical CD4+ T lymphocyte counting accuracy.

Public Health Relevance

Our objective is to develop a rapid, accurate and low-cost HIV monitoring device using a novel CD4+ T lymphocyte purification and counting technology at resource limited settings. We have been working on microfluidic CD4+ T cell counting microchips and also tested them with HIV patient samples. Here, we will leverage from this expertise to create an on-chip CD4+ T cell purification technology that can eliminate CD4+ monocytes to achieve 1 10% clinical CD4+ T lymphocyte counting accuracy.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21AI087107-01A1
Application #
7930392
Study Section
AIDS Discovery and Development of Therapeutics Study Section (ADDT)
Program Officer
Livnat, Daniella
Project Start
2010-03-01
Project End
2012-02-29
Budget Start
2010-03-01
Budget End
2011-02-28
Support Year
1
Fiscal Year
2010
Total Cost
$267,000
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115
Yildiz, U Hakan; Inci, Fatih; Wang, ShuQi et al. (2015) Recent advances in micro/nanotechnologies for global control of hepatitis B infection. Biotechnol Adv 33:178-190
Shafiee, Hadi; Kanakasabapathy, Manoj Kumar; Juillard, Franceline et al. (2015) Printed Flexible Plastic Microchip for Viral Load Measurement through Quantitative Detection of Viruses in Plasma and Saliva. Sci Rep 5:9919
Wang, ShuQi; Akbas, Ragip; Demirci, Utkan (2015) Microchip ELISA coupled with cell phone to detect ovarian cancer HE4 biomarker in urine. Methods Mol Biol 1256:111-21
Tokel, Onur; Yildiz, Umit Hakan; Inci, Fatih et al. (2015) Portable microfluidic integrated plasmonic platform for pathogen detection. Sci Rep 5:9152
Sobieranski, Antonio C; Inci, Fatih; Tekin, H Cumhur et al. (2015) Portable lensless wide-field microscopy imaging platform based on digital inline holography and multi-frame pixel super-resolution. Light Sci Appl 4:
Inci, Fatih; Filippini, Chiara; Baday, Murat et al. (2015) Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE2RD) for diagnostics. Proc Natl Acad Sci U S A 112:E4354-63
Shafiee, Hadi; Asghar, Waseem; Inci, Fatih et al. (2015) Paper and flexible substrates as materials for biosensing platforms to detect multiple biotargets. Sci Rep 5:8719
Shafiee, Hadi; Wang, ShuQi; Inci, Fatih et al. (2015) Emerging technologies for point-of-care management of HIV infection. Annu Rev Med 66:387-405
Wang, ShuQi; Tasoglu, Savas; Chen, Paul Z et al. (2014) Micro-a-fluidics ELISA for rapid CD4 cell count at the point-of-care. Sci Rep 4:3796
Tokel, Onur; Inci, Fatih; Demirci, Utkan (2014) Advances in plasmonic technologies for point of care applications. Chem Rev 114:5728-52

Showing the most recent 10 out of 40 publications