We propose to develop an alternative approach to address and treat HBV infection with targeted rare-cutting endonucleases. We will use zinc finger nucleases (ZFNs) to induce double-strand breaks within specific HBV sequences upon AAV-mediated ZFN delivery. We hope to achieve virologic cure of infected cells by promoting cellular degradation of cccDNA present in infected hepatocytes upon linearization or inactivating HBV genes within the episomal cccDNA. The repair mechanism for double-strand breaks is error prone and should result in disruption of genes around the cleaved target sequence. This proposal will study the efficacy and safety of our approach in the highly relevant humanized uPA-SCID mouse model of HBV infection. Our preliminary data shows that we can successfully mutate targeted HBV genes and inhibit HBV replication in an in vitro cell culture model of HBV using AAV-delivered ZFNs. It also shows that our HBV-specific ZFNs cause minimal levels of off-target cleavage at similar sites within the human genome. We have established the uPA-SCID mouse model of HBV infection and have demonstrated efficient delivery of a fluorescent reporter gene to human hepatocytes in humanized mouse livers using AAV vectors. Our comprehensive preliminary data provides the basis for a pilot study of the antiviral efficacy of this therapeutic approach in vivo. The overall aim of this study is to provide proof of principle fr its use in treating chronic HBV infections.

Public Health Relevance

The long-term goal of this project is to combine our team's unique expertise in rare cutting endonuclease technologies (ZFNs, TALENs and homing endonucleases), virology, gene delivery systems, and mouse models of HBV infection to generate a therapeutic approach allowing us to specifically target and disrupt hepatitis B virus genomes in livers of chronically infected individuals. This plan offers a plausible way to test the proof of principle for this therapy with the long-term aim of providing a cure for individuals infected with HBV.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21AI107252-02
Application #
9008017
Study Section
Hepatobiliary Pathophysiology Study Section (HBPP)
Program Officer
Koshy, Rajen
Project Start
2015-02-06
Project End
2017-01-31
Budget Start
2016-02-01
Budget End
2017-01-31
Support Year
2
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
078200995
City
Seattle
State
WA
Country
United States
Zip Code
98109
Stone, Daniel; Niyonzima, Nixon; Jerome, Keith R (2016) Genome editing and the next generation of antiviral therapy. Hum Genet 135:1071-82