Mycobacterium abscessus is an emerging pathogen within the rapidly growing, non-tubercular mycobacterial (NTM) species. M. abscessus can cause wound and subcutaneous infections, but the most serious infections are pulmonary, and usually seen in elderly, immunocompetent individuals with underlying lung pathologies, or tall, elderly women with low body mass. Remarkably, M. abscessus is also responsible for serious pulmonary disease in patients with cystic fibrosis or those undergoing anti-TNF? (tumor necrosis factor) therapy. M. abscessus belongs to a taxonomically contentious group M. abscessus sensu lato, which includes the pathogens M. massiliense and M. bolletii. Recent analysis has suggested that these three organisms are distinct subspecies within the abscessus species designation. For the purpose of this proposal, we will focus on M. abscessus senso stricto, otherwise known as M. abscessus subsp. abscessus. The reasons why M. abscessus has become an important NTM in human disease are not clear because M. abscessus is understudied compared to other pathogenic mycobacteria. However, several studies suggest that a morphological switch may play a role in M. abscessus pulmonary pathogenesis. Environmental isolates of M. abscessus typically form smooth colonies, while isolates from patients with deep tissue infections form rough colonies. The emergence of the rough phenotype results from mutation of genes involved with the synthesis or transport of a glycopeptidolipid (GPL) present on the outer leaflet of the cell envelope. Smooth bacteria are GPL+, form biolfilms, and can be killed after engulfment by macrophages. In contrast, the rough, GPL- bacteria do not form biofilms, and are resistant to killing within macrophages. It is hypothesized that the smooth morphotype is better suited for survival in environmental niches and for the initial colonization of the host, while the rough morphotype has a survival advantage deeper in host tissue. In this application, we propose to develop new tools to examine M. abscessus pathogenesis in pulmonary disease within the framework of this hypothesis.
Mycobacterium abscessus is an emerging pathogen within the rapidly growing, non-tubercular mycobacterial (NTM) species. M. abscessus can cause wound and subcutaneous infections, but the most serious infections are pulmonary and seen in wide variety of patients. Treatment regimens are lengthy, expensive, sometimes requiring surgery, and in many cases are not curative. The reasons why M. abscessus has become an important NTM in human disease are not clear because M. abscessus is understudied compared to other pathogenic mycobacteria. This work will aim to develop new investigative tools to help understand lung disease caused by this pathogen, potentially leading the way to new methods for prevention or therapy. The work proposed in this project would contribute to the research goals of the NIAID PA-13-303: NIH Exploratory/Developmental Research Grant Program as it is an exploratory proposal aimed at developing new tools and approaches to studying the pathogenesis of Mycobacterium abscessus, an emerging, non-tubercular mycobacteria responsible for a significant amount of human disease.
| Gregoire, Stacy A; Byam, Joel; Pavelka, Martin S (2017) galK-based suicide vector mediated allelic exchange in Mycobacterium abscessus. Microbiology : |
