An antibiotic susceptibility test determines whether or not bacterial isolates from a patient?s blood, wound specimens, or urine are susceptible to administered antibiotics. A standard antibiotic susceptibility test measures the growth of sampled bacteria in antibiotics and suffers from a long cell culture step. The lack of rapid antibiotic susceptibility tests adversely affects the treatment of bacterial infections and contributes to the increased prevalence of multidrug resistant bacteria. The focus of this R21 application is on urinary tract infections. In particular, a novel approach and device are proposed for rapidly determining the antibiotic susceptibility of bacteria in urine. Bacteria exhibit various types of movements, which appear to subside promptly upon exposure to an effective antibiotic. In the proposed study, the movements of bacteria that are causative pathogens for urinary tract infections will be measured before and after exposure to antibiotics. The magnitude of the measured bacterial movements will be correlated with antibiotic susceptibility. These measurements will be enabled by a novel tailor-made microfluidic transducer that converts bacterial movements into electrical signals with high fidelity. This device will interface with microfluidics and work with unprocessed urine samples. The proposed approach to antibiotic susceptibility testing is phenotypic, will eliminate the need for extensive culturing, and will allow for rapid testing. It is robust enough that it has the potential to be used at the point of care, when successfully developed.
The aim and scope of the proposed study are clearly defined. This collaborative team consisting of experts in physical science (Ekinci), biological science (Stearns-Kurosawa), and clinical infectious diseases (Gupta) will be able to address any unforeseen challenges. Thus, the study has an excellent chance of success.

Public Health Relevance

The goal of this project is to develop a novel test for determining the antibiotic susceptibility of bacteria causing urinary tract infections. The successful development of this innovative test will allow for rapid and efficient treatment of urinary tract infections; it will also help combat the increasing problem of antibiotic resistance in bacteria.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21AI133264-02
Application #
9700587
Study Section
Instrumentation and Systems Development Study Section (ISD)
Program Officer
Ritchie, Alec
Project Start
2018-05-22
Project End
2021-04-30
Budget Start
2019-05-01
Budget End
2021-04-30
Support Year
2
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Boston University
Department
Engineering (All Types)
Type
Biomed Engr/Col Engr/Engr Sta
DUNS #
049435266
City
Boston
State
MA
Country
United States
Zip Code
02215