There has been a tremendous increase in the use of nutraceuticals by health consumers for the treatment and prevention of a variety of medical conditions. However, there is very little information in the scientific literature regarding the efficacy and safety of these naturally occurring substances. The overall long-term objectives of the proposed research are: 1) to enhance our understanding of the mechanism of action of nutraceuticals; 2) to determine whether the use of nutraceuticals is associated with adverse effects; and 3) to elucidate the biochemical and molecular mechanisms by which the concurrent use of a nutraceutical and a drug lead to drug interactions. The ultimate aim of this research is to provide a scientific basis for the rational and safe use of nutraceutical products. Therefore, the proposed research has direct relevance to human health. The proposed experiments in the present application is to investigate the effect of ginsenosides on human CYP enzymes which are important catalysts in drug biotransformation and procarcinogen activation. Specifically, the proposed experiments will test the hypotheses that ginsenosides: 1) suppresses the inducibility of CYP1A1 and CYP1B1; 2) inhibits the catalytic activity of CYP1A1, CYP1A2, and CYP1B1; 3) inhibits the hepatic microsomal metabolism of drugs catalyzed by CYP1A2; and 4) inhibits the catalytic activity of CYP3A4 and CYP3A5. These studies will be performed in vitro by employing recombinant cytochrome P450 enzymes, human liver microsomes, and cell culture models (i.e., MCF-7 human breast carcinoma cells) of cytochrome P450.

Agency
National Institute of Health (NIH)
Institute
National Center for Complementary & Alternative Medicine (NCCAM)
Type
Exploratory/Developmental Grants (R21)
Project #
3R21AT000286-02S1
Application #
6449894
Study Section
Special Emphasis Panel (ZAT1 (03))
Program Officer
West, Neal B
Project Start
2000-07-01
Project End
2002-04-30
Budget Start
2001-05-01
Budget End
2002-04-30
Support Year
2
Fiscal Year
2001
Total Cost
$951
Indirect Cost
Name
University of British Columbia
Department
Type
DUNS #
251949962
City
Vancouver
State
BC
Country
Canada
Zip Code
V6 1-Z3