The ventral tegmental area (VTA) sends dense projections to the nucleus accumbens (NAc), constituting the backbone of the mesolimbic system. The mesolimbic system is often targeted by drugs of abuse, stress, and other severe experience to change the emotional and motivational states, resulting in a variety of psychological and psychiatric disorders. In addition to dopamine and GABA, the VTA-to-NAc projections also release glutamate. The VTA-to-NAc glutamatergic transmission has been thought to critically regulate NAc principle medium spiny neurons (MSNs), and contribute to motivated behaviors as well as behavioral alterations after exposure to drugs of abuse. However, despite extensive exploration, only weak cellular effects of the VTA-to- NAc glutamatergic signaling have been detected. For example, VTA-released glutamate evokes EPSCs in NAc MSNs as well as in interneurons, but these EPSCs are usually very small compared to EPSCs evoked from other major glutamatergic sources. A critical question is whether there are cellular mechanisms through which the VTA-to-NAc glutamatergic transmission effectively regulates NAc MSNs and NAc-based behaviors. Our preliminary results reveal a novel and robust cellular role of the VTA-to-NAc glutamatergic projection. Specifically, activation of the VTA-to-NAc glutamatergic projection transiently inhibited the responsiveness of MSNs to other major excitatory inputs, providing a potential time-locked shunting of NAc MSNs upon VTA activation. Thus, rather than a depolarization driver, the VTA-to-NAc glutamatergic projection functions as a regulator of other ongoing excitatory inputs to NAc MSNs. This regulation can be particularly important in behaving animals, in which activation of NAc MSNs must be finely controlled in response to incoming excitatory inputs to achieve select behaviors. Our subsequent preliminary results suggest a novel mechanism mediating this regulation, a mechanism involving activation of NAc astrocytes and astrocytic release of gliotransmitters. Because each astrocyte ensheathes a population of neurons, this potential effect of astrocytes may help synchronize population activities of NAc MSNs. The objective of this application is to thoroughly characterize the cellular basis of this VTA-to-NAc glutamatergic transmission-mediated regulation of NAc MSNs. Based on extensive preliminary results, we hypothesize a neuron-glia-neuron interaction mechanism: activation of the VTA-to-NAc glutamatergic projection activates metabotropic glutamate receptor 5 (mGluR5) on NAc astrocytes, which results in astrocytic release of ATP to presynaptically inhibit ongoing excitatory synaptic transmissions to NAc MSNs from other major glutamatergic inputs. By testing this hypothesis, we expect to characterize the cellular and circuitry mechanisms underlying VTA-to-NAc glutamatergic regulation. These mechanisms and involved molecular substrates may provide a conceptual and experimental foundation for future comprehensive studies to reveal the cellular, circuitry, and behavioral roles of VTA-to-NAc glutamatergic signaling, and how to manipulate this signaling to achieve clinical benefits.

Public Health Relevance

In addition to dopamine and GABA, projections from the ventral tegmental area (VTA) also release glutamate to the nucleus accumbens (NAc), but the physiological role of this glutamatergic signaling remains poorly understood. The proposed experiments will characterize a novel mechanism through which the VTA-to-NAc glutamatergic signaling regulates ongoing excitatory inputs to the NAc. The expected findings will provide a set of molecular substrates, which can be targeted to optimize the functional output of the NAc under physiological and pathophysiological conditions.

National Institute of Health (NIH)
National Institute on Drug Abuse (NIDA)
Exploratory/Developmental Grants (R21)
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Pathophysiological Basis of Mental Disorders and Addictions Study Section (PMDA)
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Sorensen, Roger
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University of Pittsburgh
Schools of Arts and Sciences
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Wang, Junshi; Ishikawa, Masago; Yang, Yue et al. (2018) Cascades of Homeostatic Dysregulation Promote Incubation of Cocaine Craving. J Neurosci 38:4316-4328
Wright, William J; Dong, Yan (2018) Intrinsic Excitability of Cocaine-Associated Memories. Neuropsychopharmacology 43:675-676
Koya, Eisuke; Dong, Yan (2018) Sound of silent synapses from the addicted hippocampus. Neuropsychopharmacology 43:1981-1982
Cahill, Michael E; Browne, Caleb J; Wang, Junshi et al. (2018) Withdrawal from repeated morphine administration augments expression of the RhoA network in the nucleus accumbens to control synaptic structure. J Neurochem 147:84-98
Labonté, Benoit; Engmann, Olivia; Purushothaman, Immanuel et al. (2017) Sex-specific transcriptional signatures in human depression. Nat Med 23:1102-1111
Dong, Yan; Taylor, Jane R; Wolf, Marina E et al. (2017) Circuit and Synaptic Plasticity Mechanisms of Drug Relapse. J Neurosci 37:10867-10876
Wright, William J; Dong, Yan (2017) Tipping the Scales Toward Addiction. Biol Psychiatry 81:903-904
Yu, Jun; Yan, Yijin; Li, King-Lun et al. (2017) Nucleus accumbens feedforward inhibition circuit promotes cocaine self-administration. Proc Natl Acad Sci U S A 114:E8750-E8759
Wright, William J; Dong, Yan (2016) N-Methyl-D-Aspartate Receptors: ""C""ing the Culprits Behind Cocaine-Induced Metaplasticity. Biol Psychiatry 80:644-646