Abstract

The practical goals of this study will be the development and optimization of a serum free cryoprotection medium with minimum levels of cryoprotectants. We intend to pursue these studies using a hypothesis-driven, mechanism-based experimental approach, i.e., the viability of the cryopreserved adult stem cells (ASCs), in the undifferentiated, adipocyte- or osteoblast- differentiated states, can be improved by increasing the pre-freeze expression of heat shock proteins (HSPs).

Public Health Relevance

This proposal will determine the link between the expression of heat shock proteins and pre-freeze (control) and post-freeze (cryopreservation) potential (osteogenic and adipogenic) of human adipose tissue derived adult stem cells (ASCs).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21DK091852-02
Application #
8441521
Study Section
Cellular Aspects of Diabetes and Obesity Study Section (CADO)
Program Officer
Haft, Carol R
Project Start
2012-07-01
Project End
2014-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
2
Fiscal Year
2013
Total Cost
$178,525
Indirect Cost
$46,320

  Institution

Name
Louisiana State University A&M Col Baton Rouge
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803

  Publications

Shaik, Shahensha; Wu, Xiying; Gimble, Jeffrey et al. (2018) Effects of Decade Long Freezing Storage on Adipose Derived Stem Cells Functionality. Sci Rep 8:8162
Mehta, Nishir; Shaik, Shahensha; Devireddy, Ram et al. (2018) Single-Cell Analysis Using Hyperspectral Imaging Modalities. J Biomech Eng 140:
Devireddy, Ram V (2013) BIOPRESERVATION: HEAT/MASS TRANSFER CHALLENGES AND BIOCHEMICAL/GENETIC ADAPTATIONS IN BIOLOGICAL SYSTEMS. Heat Transf Res 44:245-272