We propose pioneering research investigating the synthesis and development of novel hybrid materials, as well as the development of innovative photo catalytic techniques for patterning surfaces using the novel materials. We seek to pattern surface arrays having nanometer-scale features that are smaller than the wavelength of incident light, using inexpensive materials and repeatable methods. It is our hypothesis that activation of a stamp-bound photocatalyst by a relatively broad band light source can be employed to selectively modify surface bound chemistries in close apposition to the photocatalyst and in regions much smaller than the wavelength of light. Such modification of a previously designed chemistry would change non-adhesive substrate regions to adhesive regions, thus encouraging the retention of DNA, proteins, and cells. The long-term goal of this research is to deterministically collect and organize proteins and DNA into nanoarrays on otherwise biocompatible, non-adhesive surfaces for bio-detection, high-throughput assays and crystal structure determination. The primary deliverables for this proposal are as follows: 1. Establish a robust platform to homogeneously couple a photocatalyst to a transparent, soft material 2. Measure and document interracial characteristics of photocatalytic hybrid stamps 3. Optimize excitation parameters for photocatalytic oxidation 4. Document repeatable surface patterning of cells using photocatalysis and biocompatible surface chemistries 5. Demonstrate nanometer scale surface patterning with proteins and DNA

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21EB003991-02
Application #
6944354
Study Section
Special Emphasis Panel (ZRG1-ISD (01))
Program Officer
Korte, Brenda
Project Start
2004-09-01
Project End
2007-08-31
Budget Start
2005-09-01
Budget End
2007-08-31
Support Year
2
Fiscal Year
2005
Total Cost
$246,642
Indirect Cost
Name
Lawrence Livermore National Laboratory
Department
Physics
Type
Organized Research Units
DUNS #
827171463
City
Livermore
State
CA
Country
United States
Zip Code
94550
Bearinger, Jane P; Stone, Gary; Dugan, Lawrence C et al. (2009) Porphyrin-based photocatalytic nanolithography: a new fabrication tool for protein arrays. Mol Cell Proteomics 8:1823-31
Bearinger, Jane; Dugan, Lawrence; Wu, Ligang et al. (2009) Chemical tethering of motile bacteria to silicon surfaces. Biotechniques 46:209-16
Bearinger, Jane P; Stone, Gary; Hiddessen, Amy L et al. (2009) Phototocatalytic lithography of poly(propylene sulfide) block copolymers: toward high-throughput nanolithography for biomolecular arraying applications. Langmuir 25:1238-44
Bearinger, Jane P; Stone, Gary; Christian, Allen T et al. (2008) Porphyrin-based photocatalytic lithography. Langmuir 24:5179-84
Feller, L; Bearinger, J P; Wu, L et al. (2008) MICROPATTERNING OF GOLD SUBSTRATES BASED ON POLY(PROPYLENE SULFIDE-BL-ETHYLENE GLYCOL), (PPS-PEG) BACKGROUND PASSIVATION AND THE MOLECULAR-ASSEMBLY PATTERNING BY LIFT-OFF (MAPL) TECHNIQUE. Surf Sci 602:2305-2310