Transcranial Direct Current Stimulation (tDCS) is a non-invasive electrical stimulation technique investigated for a broad range of medical and performance indications. Understanding the cellular mechanisms of tDCS will increase the rigor of ongoing studies and provide a rational basis for dose optimization. Prior mechanistic studies have focused exclusively on direct polarization of neuronal membranes by direct current stimulation (DCS). We propose to test the hypothesis that tDCS directly and transiently modulates blood-brain-barrier (BBB) function, which in turn would modulate neuronal activity. Our approach is to use state-of-the-art animal and tissue models and characterization to determine if a new-class of cellular targets, namely endothelial cells, respond to DCS. These approaches including multi-photon transcranial quantitative imaging of vascular permeability during and after DCS and isolation of molecular and generic responses of endothelial barriers. Because understanding every cellular target of stimulation is required for a comprehensive mechanism, the modulation of BBB by tDCS, in conjunction with direct neuronal effects, is novel and critical to research. This study will be the first to establish the feasibility of direct BBB actions by tDCS as well as quantitatively predict the impact of these changes on neuronal function.

Public Health Relevance

Transcranial direct current stimulation (tDCS) is a novel technique using two electrodes on the head and weak applied current. This study will determine if tDCS acts not only on neurons, but also on endothelial cells in the BBB barrier. Understanding the cellular targets of tDCS will help improved the efficacy and specificity of this technique.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Exploratory/Developmental Grants (R21)
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Special Emphasis Panel (BNVT)
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Peng, Grace
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City College of New York
Engineering (All Types)
Schools of Engineering
New York
United States
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Khadka, Niranjan; Zannou, Adantchede L; Zunara, Fatima et al. (2018) Minimal Heating at the Skin Surface During Transcranial Direct Current Stimulation. Neuromodulation 21:334-339
Cancel, Limary M; Arias, Katherin; Bikson, Marom et al. (2018) Direct current stimulation of endothelial monolayers induces a transient and reversible increase in transport due to the electroosmotic effect. Sci Rep 8:9265
Jackson, Mark P; Bikson, Marom; Liebetanz, David et al. (2017) How to consider animal data in tDCS safety standards. Brain Stimul 10:1141-1142
Jackson, Mark P; Truong, Dennis; Brownlow, Milene L et al. (2017) Safety parameter considerations of anodal transcranial Direct Current Stimulation in rats. Brain Behav Immun 64:152-161
Jackson, Mark P; Bikson, Marom; Liebetanz, David et al. (2017) Toward comprehensive tDCS safety standards. Brain Behav Immun 66:413
Chrysikou, Evangelia G; Berryhill, Marian E; Bikson, Marom et al. (2017) Editorial: Revisiting the Effectiveness of Transcranial Direct Current Brain Stimulation for Cognition: Evidence, Challenges, and Open Questions. Front Hum Neurosci 11:448
Jackson, Mark P; Rahman, Asif; Lafon, Belen et al. (2016) Animal models of transcranial direct current stimulation: Methods and mechanisms. Clin Neurophysiol 127:3425-3454
Bikson, Marom; Grossman, Pnina; Thomas, Chris et al. (2016) Safety of Transcranial Direct Current Stimulation: Evidence Based Update 2016. Brain Stimul 9:641-661
Khadka, Niranjan; Rahman, Asif; Sarantos, Chris et al. (2015) Methods for specific electrode resistance measurement during transcranial direct current stimulation. Brain Stimul 8:150-9