Plasticity is a consequence of environmental exposures during critical life periods affecting physiological systems orchestrating underlying developmental processes. The autonomic nervous system (ANS) is a putative key regulatory system vulnerable to perinatal environmental programming toward trajectories of enhanced pediatric and adult disease risk. Epidemiological studies that examine candidate environmental factors that program the ANS starting in utero and explore putative underlying mechanisms are scarce. While environmental influences are diverse, factors gaining attention with respect to fetal programming and autonomic disruption include particulate air pollution and maternal diet (e.g., oxidant/antioxidant balance). While these factors have generally been considered independently, evolving theory underscores the importance of studying their integrated effects. Biological mechanisms responsible for adverse outcomes associated with particulate pollution include ANS imbalance and the induction of oxidative stress is thought to have a central role. Air pollution effects on the mother during pregnancy may disrupt fetal-placental oxidant/antioxidant balance with implications for the developing ANS. Dietary factors may play a modulating role on effects of air pollutants. This investigative team leveraged the Harvard Programming of Intergenerational Stress Mechanisms (PRISM) study, a prenatal urban cohort, to add nutritional measures to study and examine associations among prenatal short-term (7-day mean) and chronic exposure to ambient particulate pollution [PM2.5, black carbon (BC) as a marker of traffic-related particles] and autonomic function in 6-month old infants. ANS phenotyping is indexed through a neuroendocrinologial approach analyzing salivary -amylase levels during basal ambulatory conditions and following an in-laboratory stressor. An electrophysiological approach is also employed to analyze ANS reactivity [indexed by respiratory sinus arrhythmia (RSA)] in response to the same in-laboratory stressor. Secondly, associations among long- and short- term prenatal ambient particulate pollution, biomarkers of oxidative stress (F2-isoprostanes) will be examined in maternal prenatal urine and cord blood, and infant autonomic functioning to explore whether associations between prenatal pollution and infant autonomic balance are mediated through enhanced oxidative stress. Finally, ambient particulate pollution will be examined on ANS functioning in 6-month olds to see if the effects are modified by maternal prenatal dietary factors that may protect the infant from oxidative stress [e.g., ?-3 fatt acids and antioxidant micronutrients (e.g., vitamins C and E and flavonoids)] ascertained using a triad approach incorporating a food frequency questionnaire (FFQ), dietary records obtained twice during pregnancy, and select maternal serum/plasma biomarkers ascertained prenatally.

Public Health Relevance

Our findings will provide insight into the possible role of particulate air pollution and prenatal maternal diet in the programming of autonomic balance. Given the generalized integration of autonomic nervous system (ANS) functioning (e.g.; involvement in the regulation of cardiovascular functioning as well as modulation of the immune system; inflammation; and metabolism); disruption of autonomic balance in early development may have broad long- term health implications. Identifying specific chemical and nutritional programming agents that are amenable to intervention; and understanding the mechanisms by which they exert their effects during developmentally sensitive periods; may enhance our understanding of the origins of many disorders and suggest more efficacious preventative strategies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Exploratory/Developmental Grants (R21)
Project #
7R21ES021318-02
Application #
8574006
Study Section
Special Emphasis Panel (ZES1-LWJ-J (DI))
Program Officer
Gray, Kimberly A
Project Start
2012-08-10
Project End
2015-05-31
Budget Start
2012-08-10
Budget End
2013-05-31
Support Year
2
Fiscal Year
2012
Total Cost
$183,546
Indirect Cost
$72,830
Name
Icahn School of Medicine at Mount Sinai
Department
Type
Schools of Medicine
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
Sheffield, Perry E; Speranza, Rosa; Chiu, Yueh-Hsiu Mathilda et al. (2018) Association between particulate air pollution exposure during pregnancy and postpartum maternal psychological functioning. PLoS One 13:e0195267
Bose, Sonali; Rosa, Maria José; Mathilda Chiu, Yueh-Hsiu et al. (2018) Prenatal nitrate air pollution exposure and reduced child lung function: Timing and fetal sex effects. Environ Res 167:591-597
Rosa, Maria José; Pajak, Ashley; Just, Allan C et al. (2017) Prenatal exposure to PM2.5 and birth weight: A pooled analysis from three North American longitudinal pregnancy cohort studies. Environ Int 107:173-180
Lipton, L R; Brunst, K J; Kannan, S et al. (2017) Associations among prenatal stress, maternal antioxidant intakes in pregnancy, and child temperament at age 30 months. J Dev Orig Health Dis 8:638-648
Brunst, Kelly J; Kannan, Srimathi; Ni, Yu-Ming et al. (2016) Validation of a Food Frequency Questionnaire for Estimating Micronutrient Intakes in an Urban US Sample of Multi-Ethnic Pregnant Women. Matern Child Health J 20:250-60
Brunst, Kelly J; Enlow, Michelle Bosquet; Kannan, Srimathi et al. (2014) Effects of Prenatal Social Stress and Maternal Dietary Fatty Acid Ratio on Infant Temperament: Does Race Matter? Epidemiology (Sunnyvale) 4:
Brunst, Kelly J; Wright, Robert O; DiGioia, Kimberly et al. (2014) Racial/ethnic and sociodemographic factors associated with micronutrient intakes and inadequacies among pregnant women in an urban US population. Public Health Nutr 17:1960-70