During the formation of the lens, a transient vessel network, the hyaloid network, forms to nourish the lens as it develops. However, during late fetal development in humans and early postnatal development in mice, hyaloid vessels undergo regression to enable a transparent path of vision through the lens. Abnormal regression or growth of the hyaloid vasculature results in several diseases, including persistent hyperplastic tunica vasculosa lentis (PHTVL), persistent hyperplastic primary vitreous (PHPV), persistent fetal vasculature (PFV) and persistent prepupillary membrane (PPM). In this study, we will use fluorescent reporter mice labeling key cell types involved in vessel regression, live confocal imaging and FACS sorting and expression analysis to define cellular events leading to the onset of vessel regression.

Public Health Relevance

The abnormal growth and regression of blood vessels in the eye is the leading cause of blindness in children, adults and the elderly (Campochiaro, 2000). This study will use vital imaging to characterize how vessels regress in the newborn eye using mouse models in which individual cell types in the hyaloid vasculature are labeled with fluorescent protein reporters. Live imaging and FACS sorting will be used to define novel events in that initiate vessel regression.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21EY020632-01
Application #
7875268
Study Section
Anterior Eye Disease Study Section (AED)
Program Officer
Araj, Houmam H
Project Start
2010-05-01
Project End
2012-04-30
Budget Start
2010-05-01
Budget End
2011-04-30
Support Year
1
Fiscal Year
2010
Total Cost
$191,875
Indirect Cost
Name
Baylor College of Medicine
Department
Physiology
Type
Schools of Medicine
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
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