The single aim is to develop a system of meiotic genetic analysis for Dictyostelium discoideum. Dictyostelium has served as a model system for studying single cell amoeboid motility and chemotaxis and multi- cellular fate selection and regulation. The major limitation has been the difficulty in genetic analysis of this haploid organism. Dictyostelium has enjoyed a resurgence in recent years due the ease of homologous gene replacement to study a variety of cellular functions. In the wild, Dictyostelium has a sexual, diploid cycle. Unfortunately, the laboratory strains of Dictyostelium have lost this ability. Kessin proposes methods to establish sexual strains of Dictyostelium which will be essential for genetic mapping, isogenization of strains, and creating multiple mutant stocks.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21GM056360-02
Application #
6019338
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1998-09-30
Project End
2000-08-31
Budget Start
1999-09-01
Budget End
2000-08-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Pathology
Type
Schools of Medicine
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032