This is a proposal to establish the technological proof-of-principle for a high-throughput and parallizable means for generating fully human monoclonal antibodies. We describe the development of a new version of the yeast two-hybrid (Y2H) system to identify antibodies that interact with a bait protein, a novel iterative method for increased searching of sequence space, and a method with which to quickly, cheaply and accurately identify specific antibody sequences giving rise to antibody-antigen interactions. The antibodies we derive are designed to be cloned into frameworks for the immediate development of clinically useful antibody drugs. ? ?

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21HG003327-01
Application #
6815043
Study Section
Genome Study Section (GNM)
Program Officer
Felsenfeld, Adam
Project Start
2004-09-24
Project End
2006-08-31
Budget Start
2004-09-24
Budget End
2005-08-31
Support Year
1
Fiscal Year
2004
Total Cost
$125,000
Indirect Cost
Name
Rothberg Institute, Inc.
Department
Type
DUNS #
085499809
City
Guilford
State
CT
Country
United States
Zip Code
06437