The human gut microbiota makes important functional contributions to the host's metabolism and physiological traits, but remains largely unknown due to its complexity. To generate DNA materials of the microbial species from the human microbiota suitable for genomic sequencing, we propose to design, construct and test droplet based microfluidic devices for the co-cultivation and analysis of various subsets of the total gut microbiota. We will pursue two aims:
Specific Aim 1 : Design and construct microfluidic components for cell encapsulation, co-cultivation, and genetic characterization.
Specific Aim 2 : Test the devices with a synthetic model system and gut microbial samples from gnotobiotic animals.

Public Health Relevance

Microbial communities in the gastrointestinal tract have been found to make functional contributions to the host's metabolism and physiological traits, such as digestion and immunity. To facilitate the cultivation of these gut microbes and to eventually elucidate the underlying microbe-microbe and host-microbe interactions, we propose to design and build a prototype microfluidic platform to compartmentalize, co-cultivate and analyze various subsets of the total gut microbiota.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21HG005077-02
Application #
8150950
Study Section
Special Emphasis Panel (ZRG1-IDM-M (50))
Program Officer
Schloss, Jeffery
Project Start
2010-09-27
Project End
2013-12-31
Budget Start
2011-07-01
Budget End
2013-12-31
Support Year
2
Fiscal Year
2011
Total Cost
$215,879
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Park, Jihyang; Kerner, Alissa; Burns, Mark A et al. (2011) Microdroplet-enabled highly parallel co-cultivation of microbial communities. PLoS One 6:e17019