Studying the Glial Contribution to RTT Pathogenesis Using Patient-Specific iPSCs Abstract Rett syndrome (RTT) is an autism spectrum disorder (ASD) that predominantly affects females. The identification of mutations in the X-linked MECP2 (methyl-CpG binding protein 2) gene as the cause of RTT has led to the creation of mouse models for studying disease mechanism. However, mouse models have limitations in mimicking human RTT mutations and in drug screening. Induced pluripotent stem cells (iPSCs) have been generated from RTT patients as an in vitro human model to validate and extend knowledge obtained from mouse studies. Currently, all studies involving RTT iPSCs have focused on studying neuronal pathologies in the absence of astrocytes. As the other major cell type in the brain, astrocytes also express MeCP2. There is strong evidence, from work in RTT mouse models, that astrocytes play a critical role in disease progression. Thus, we hypothesize that astrocytes differentiated from mutant RTT-iPSC lines will significantly impair neuronal growth and maturation when cultured together with neurons. In the current proposal, we will focus our attention on revealing the glial contribution to RTT pathology in a neuron/astrocyte co-culture system. To minimize phenotypic variation caused by different genetic backgrounds across iPSC lines generated from different individuals, we have established several pairs of isogenic iPSC lines from the same female RTT patients (carrying either common or rare RTT mutations) skin cells that clonally express either the wild type copy or the mutant copy (but not both) of the MECP2 gene. Using these unique tools, we plan to test 1) whether mutant iPSC-derived astrocytes may impair the growth and maturation of wild type iPSC-derived neurons in the neuron/astrocyte co-culture;2) whether wild type iPSC-derived astrocytes may rescue the growth and maturation defects of mutant type iPSC-derived neurons in the neuron/astrocyte co-culture;and 3) whether the potential astrocyte influnce is mediated by cell-cell contact or secreted molecules. Better understanding of the glial contribution to RTT pathology will not only provide insight into disease mechanisms, but also lay the groundwork for future drug screens using RTT iPSC-derived neurons and/or astrocytes. Furthermore, in light of the recent evidence that MECP2 may be altered at both the genomic level and the expression level in many autism patients, the lessons learned and the experimental approaches used in studying RTT might also benefit the general understanding of autism.
Studying the Glial Contribution to RTT Pathologenesis Using Patient-Specific iPSCs Project Narrative Mutations in the X-linked human MECP2 gene (methyl-CpG binding protein 2) cause Rett syndrome (RTT), an autism spectrum developmental disorder that predominantly affects females. To fully understand the molecular mechanism of RTT, it is important to study the role of astrocytes in disease progression in human cells. The successful completion of this project will significantly advance the understanding of RTT disease mechanism and facilitate future development of therapies to treat RTT. First, the proposal aims at studying RTT pathogenesis in human RTT neurons and astrocytes, the two major cell types affected in RTT patients. This is necessary because mice are not humans. Any knowledge obtained in mouse studies or any treatment showing efficacy in mouse models should be validated in human systems. Second, the proposal aims at revealing the glial contribution to RTT pathology, which will open up a research area that has not been explored in human cells. This is very important for treating RTT, because strategies aimed at correcting neuronal defects may not work efficiently if glial defects are left untreated. Third, the proposal will establish RT induced pluripotent stem cell (iPSC)-derived neurons and astrocytes as in vitro platforms for future drug screens, which is the next step to translate discoveries at the bench side to treatments at the bed side. Finally, because of the considerable overlap in clinical features between RTT and autism spectrum disorders and the recent reports of MECP2 alterations in autism patients, the lessons learned studying RTT might also benefit the general understanding of autism.
| Dong, Qiping; Liu, Qing; Li, Ronghui et al. (2018) Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes. Elife 7: |
| Williams, Emily Cunningham; Zhong, Xiaofen; Mohamed, Ahmed et al. (2014) Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons. Hum Mol Genet 23:2968-80 |
| Musah, Samira; Wrighton, Paul J; Zaltsman, Yefim et al. (2014) Substratum-induced differentiation of human pluripotent stem cells reveals the coactivator YAP is a potent regulator of neuronal specification. Proc Natl Acad Sci U S A 111:13805-10 |
