Complement promotes synaptic pruning by glia in order to refine neural circuits during development, but may also pathologically destroy mature, essential synapses in the context of neuroinflammation. Immune dysfunction and an imbalance in synaptic pruning have been implicated in autism spectrum disorder (ASD), and recent studies suggest that dysregulation of complement may be connected. The central hypothesis to be tested in this application is that the Sez6 gene family, whose members have been identified as ASD susceptibility genes, are novel, synaptically localized, complement regulators that are required to prevent aberrant synaptic pruning related to ASD.
In Aim 1, we will define the complement regulatory properties of Sez6, Sez6L, and Sez6L2 using standard complement assays. We will then investigate whether the Sez6 family can prevent complement deposition at synapses in vitro.
In Aim 2, we will use the 16p11.2 deletion mouse model of autism, in which Sez6L2 is lost along with other genes, to determine if enhanced complement dependent synapse pruning occurs at retinogeniculate synapses in the visual system. A Sez6L2 knockout will also be tested in order to validate findings and confirm the importance of SezL2 to the 16p11.2 phenotype. In aggregate, we expect the data obtained from these experiments to advance our understanding of the role of Sez6 proteins in mechanisms underlying complement-mediated synapse elimination during development that may explain how this gene family contributes susceptibility to ASD.

Public Health Relevance

Connections between neurons are sculpted during brain development by the complement system, a network of proteins that normally works with immune cells throughout the body to help fight infection and cleanup damaged tissue. We are investigating the function of the Sez6 gene family to determine if it controls abnormal complement activation in the brain and whether this contributes to the incorrect connections between nerve cells that can give rise to autism spectrum disorders (ASD). By understanding how this contributes to ASD we hope to design treatments in the future that repair this pathway to protect the brain from ASD.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21NS111255-02
Application #
9891120
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Mamounas, Laura
Project Start
2019-04-01
Project End
2021-03-31
Budget Start
2020-04-01
Budget End
2021-03-31
Support Year
2
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Rochester
Department
Type
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627