? The goal of this work is-to develop mass spectrometry capabilities and techniques to determine the secondary and tertiary'structure of biomolecules. The initial part of the work will involve modifying a sector mass spectrometer by adding a high-field asymmetric waveform ion mobility spectrometer/quadrupole mass filter to the front end. In the interface between the quadrupole and sector portions of the instrument will be several reaction regions in which low or and high energy collision-induced dissociation (CID), and electron capture induced dissociation. The primary area of investigation will involve determining the ability to obtain secondary (and perhaps tertiary) structure from CID. These experiments will involve the combination of ion mobility and H/D exchange with CID. These experiments will be complemented with condensed phase experiments in an attempt to determine if there is a correlation between structures in solution and in the gas phase. ? ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21RR020207-02
Application #
7286860
Study Section
Special Emphasis Panel (ZRR1-BT-6 (01))
Program Officer
Friedman, Fred K
Project Start
2006-09-15
Project End
2008-12-31
Budget Start
2007-07-01
Budget End
2008-12-31
Support Year
2
Fiscal Year
2007
Total Cost
$161,239
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599