Abstract

The objectives of the proposed study is to exploit the technology of immunoelectron microscopy to study various subcellular organelles and their function. In addition, the applicant proposes to study the plasmodial heat shock proteins and ookinete invasion of the mosquito midgut. The morphological changes of desferrioximine on the growth and development of malaria parasites will also be investigated. The pursuit of these studies will require the refinement and/or development of suitable immunoelectron microscopic techniques to prepare plasmodial preparations which retain antigenicity and ultrastructure. Such technology is required to study the subcellular structure and function of parasite specific organelles, as well as the subcellular localization of various plasmodial proteins such as the heat shock protein hsp 70 and glucose regulated proteins (grp 75.) In addition the effect of desferrioximine on in vitro and in vivo derived parasites will be determined to ascertain its usefulness in the treatment of cerebral malaria. The studies are aimed at providing a better understanding of the host parasite interaction and the subcellular level.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
2R22AI010645-19
Application #
3566203
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1978-09-29
Project End
1994-08-31
Budget Start
1990-09-01
Budget End
1991-08-31
Support Year
19
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Klotz, F W; Cohen, S J; Szarfman, A et al. (1996) Cross-reactive epitope among proteins in Plasmodium falciparum Maurer's clefts and primate leukocytes and platelets. Am J Trop Med Hyg 54:655-9
Alano, P; Read, D; Bruce, M et al. (1995) COS cell expression cloning of Pfg377, a Plasmodium falciparum gametocyte antigen associated with osmiophilic bodies. Mol Biochem Parasitol 74:143-56
Vernick, K D; Fujioka, H; Seeley, D C et al. (1995) Plasmodium gallinaceum: a refractory mechanism of ookinete killing in the mosquito, Anopheles gambiae. Exp Parasitol 80:583-95
Charoenvit, Y; Mellouk, S; Sedegah, M et al. (1995) Plasmodium yoelii: 17-kDa hepatic and erythrocytic stage protein is the target of an inhibitory monoclonal antibody. Exp Parasitol 80:419-29
Kaidoh, T; Nath, J; Fujioka, H et al. (1995) Effect and localization of trifluralin in Plasmodium falciparum gametocytes: an electron microscopic study. J Eukaryot Microbiol 42:61-4
Sam-Yellowe, T Y; Fujioka, H; Aikawa, M et al. (1995) Plasmodium falciparum rhoptry proteins of 140/130/110 kd (Rhop-H) are located in an electron lucent compartment in the neck of the rhoptries. J Eukaryot Microbiol 42:224-31
Shahabuddin, M; Kaidoh, T; Aikawa, M et al. (1995) Plasmodium gallinaceum: mosquito peritrophic matrix and the parasite-vector compatibility. Exp Parasitol 81:386-93
Ward, G E; Fujioka, H; Aikawa, M et al. (1994) Staurosporine inhibits invasion of erythrocytes by malarial merozoites. Exp Parasitol 79:480-7
Bruce, M C; Carter, R N; Nakamura, K et al. (1994) Cellular location and temporal expression of the Plasmodium falciparum sexual stage antigen Pfs16. Mol Biochem Parasitol 65:11-22
Fujioka, H; Millet, P; Maeno, Y et al. (1994) A nonhuman primate model for human cerebral malaria: rhesus monkeys experimentally infected with Plasmodium fragile. Exp Parasitol 78:371-6

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