The World Reference Center for Arvoviruses provides antigenic identification and biological characterization of viruses submitted to the the Yale Arbovirus Research Unit, designated by WHO as a Collaborating Centre. Between 100 and 200 viral strains are submitted for identification each year to the Center by field laboratories throughout the world. Directly related to this objective is the development of a systematic arrangement of the arboviruses within the higher classification of all viruses. This involves the placement of the arboviruses within recognized taxa based on morphology, morphogenesis, and physicochemical properties, and the establishment of new taxa when indicated. New techniques and methods are developed for the implementation of serological and physicochemical characterization of arboviruses. These will include polyacrylamide gel electrophoresis, monoclonal antibody, enzyme-linked immunosorbent assay, enzyme-linked fluorescent assay, and spot slide techniques among others. A bank of reagents to over 500 arboviruses is available at the Center which offers a unique diagnostic service for the recognition of etiological agents in epidemics and in clinically suspected arboviral infections of man or animals. This may involve virus isolation and identification, serological examination of paired sera or, occasionally, serosurveys. The final objectives are the training of personnel from laboratories throughout the world in arbovirus techniques; the distribution of reagents including seed virus, antigens, antibody, and tissue cultures to qualified laboratories; and the dissemination of information on arboviruses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI010984-15
Application #
3444430
Study Section
Virology Study Section (VR)
Project Start
1977-06-01
Project End
1987-05-31
Budget Start
1986-06-01
Budget End
1987-05-31
Support Year
15
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Yale University
Department
Type
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520
Greene, Ivorlyne P; Paessler, Slobodan; Anishchenko, Michael et al. (2005) Venezuelan equine encephalitis virus in the guinea pig model: evidence for epizootic virulence determinants outside the E2 envelope glycoprotein gene. Am J Trop Med Hyg 72:330-8
Greene, Ivorlyne P; Paessler, Slobodan; Austgen, Laura et al. (2005) Envelope glycoprotein mutations mediate equine amplification and virulence of epizootic venezuelan equine encephalitis virus. J Virol 79:9128-33
Holbrook, Michael R; Shope, Robert E; Barrett, Alan D T (2004) Use of recombinant E protein domain III-based enzyme-linked immunosorbent assays for differentiation of tick-borne encephalitis serocomplex flaviviruses from mosquito-borne flaviviruses. J Clin Microbiol 42:4101-10
Moncayo, Abelardo C; Fernandez, Zoraida; Ortiz, Diana et al. (2004) Dengue emergence and adaptation to peridomestic mosquitoes. Emerg Infect Dis 10:1790-6
Ratterree, Marion S; da Rosa, Amelia P A Travassos; Bohm Jr, Rudolf P et al. (2003) West Nile virus infection in nonhuman primate breeding colony, concurrent with human epidemic, southern Louisiana. Emerg Infect Dis 9:1388-94
Xiao, Shu-Yuan; Guzman, Hilda; da Rosa, Amelia P A Travassos et al. (2003) Alteration of clinical outcome and histopathology of yellow fever virus infection in a hamster model by previous infection with heterologous flaviviruses. Am J Trop Med Hyg 68:695-703
Fisher, Ann F; Tesh, Robert B; Tonry, Jessica et al. (2003) Induction of severe disease in hamsters by two sandfly fever group viruses, Punta toro and Gabek Forest (Phlebovirus, Bunyaviridae), similar to that caused by Rift Valley fever virus. Am J Trop Med Hyg 69:269-76
Beasley, David W C; Davis, C Todd; Guzman, Hilda et al. (2003) Limited evolution of West Nile virus has occurred during its southwesterly spread in the United States. Virology 309:190-5
Solomon, Tom; Fisher, Ann F; Beasley, David W C et al. (2003) Natural and nosocomial infection in a patient with West Nile encephalitis and extrapyramidal movement disorders. Clin Infect Dis 36:E140-5
Paessler, Slobodan; Fayzulin, Rafik Z; Anishchenko, Michael et al. (2003) Recombinant sindbis/Venezuelan equine encephalitis virus is highly attenuated and immunogenic. J Virol 77:9278-86

Showing the most recent 10 out of 118 publications