Abstract

Research on the """"""""genetics of the rotaviruses"""""""" will focus on two main areas: 1) Genetic analysis--A large number of temperature-sensitive (ts) mutants will be isolated from the model rotaviruses SA11 and NCDV. The ts mutants will be placed into genetic groups by complementation and recombination tests. The physiological properties and the various mutant groups will be examined, with specific emphasis on the synthesis and control of single-stranded and double-stranded RNA, polypeptides, morphogenesis and virion transcriptase. Recombinants segregating the genome segments of the parents will be isolated from crosses of ts mutants of SA11 and NCDV. The segregation patterns of the genome segments in these recombinants will be used to map ts lesions to specific genome segments and establish a map of genes encoding identical function in the two viruses. The polypeptides synthesized by the recombinants will be examined to establish the coding relationships between specific polypeptide species and specific genome segments. 2) Analysis of rotavirus biological properties and pathogenesis--Recombinants segregating the genes of SA11 and NCDV will be used to establish the genes encoding the viral neutralization antigen and the viral hemagglutinin. The virulence of the gastroenteritis produced by SA11 and NCDV will be examined in the mouse model system and the gene(s) encoding virulence factors will be identified using recombinants segregating the genes of SA11 and NCDV. Neurotropism of SA11 and NCDV in the mouse will be investigated and the gene(s) encoding the neurotropic character identified by analyzing the neurotropism of recombinants segregating the genes of the two viruses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI016687-07
Application #
3444518
Study Section
Virology Study Section (VR)
Project Start
1980-03-01
Project End
1988-02-29
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Ramig, Robert F (2007) Systemic rotavirus infection. Expert Rev Anti Infect Ther 5:591-612
Pesavento, Joseph B; Billingsley, Angela M; Roberts, Ed J et al. (2003) Structures of rotavirus reassortants demonstrate correlation of altered conformation of the VP4 spike and expression of unexpected VP4-associated phenotypes. J Virol 77:3291-6
Crawford, S E; Mukherjee, S K; Estes, M K et al. (2001) Trypsin cleavage stabilizes the rotavirus VP4 spike. J Virol 75:6052-61
Ciarlet, M; Crawford, S E; Barone, C et al. (1998) Subunit rotavirus vaccine administered parenterally to rabbits induces active protective immunity. J Virol 72:9233-46
Wentz, M J; Patton, J T; Ramig, R F (1996) The 3'-terminal consensus sequence of rotavirus mRNA is the minimal promoter of negative-strand RNA synthesis. J Virol 70:7833-41
Shaw, A L; Rothnagel, R; Zeng, C Q et al. (1996) Rotavirus structure: interactions between the structural proteins. Arch Virol Suppl 12:21-7
Zeng, C Q; Wentz, M J; Cohen, J et al. (1996) Characterization and replicase activity of double-layered and single-layered rotavirus-like particles expressed from baculovirus recombinants. J Virol 70:2736-42
Patton, J T; Wentz, M; Xiaobo, J et al. (1996) cis-Acting signals that promote genome replication in rotavirus mRNA. J Virol 70:3961-71
Wentz, M J; Zeng, C Q; Patton, J T et al. (1996) Identification of the minimal replicase and the minimal promoter of (-)-strand synthesis, functional in rotavirus RNA replication in vitro. Arch Virol Suppl 12:59-67
Zeng, C Q; Labbe, M; Cohen, J et al. (1994) Characterization of rotavirus VP2 particles. Virology 201:55-65

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