The objectives of the proposed research are to isolate and characterize--physico-chemically and immunologically--cholera related heat-labile enterotoxins (LT) from E. coli strains which produce diarrheal disease in man and animals. In recent previous work, we have isolated, for the first time, E. coli LT from a K-12 strain infected with a tox plasmid of porcine origin. The homogeneous enterotoxin was similar, but not identical, to choleragen (the cholera enterotoxin) structurally and immunologically. Partial amino acid sequence determination revealed that 15 of the first 20 N-terminal amino acids were identical in both toxins. The toxin had been purified by exploiting its unique affinity for agarose containing gels. These observations will be extended with additional strains of E. coli of human and animal origins. The immunologic and structural relationships of these toxins will be compared.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI016776-07
Application #
3444521
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1979-09-01
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Missouri-Columbia
Department
Type
Schools of Medicine
DUNS #
112205955
City
Columbia
State
MO
Country
United States
Zip Code
65211
Qu, Z H; Finkelstein, R A (1993) Checkerboard immunoblotting recognizes twenty epitopes among the B subunit proteins of the cholera enterotoxin family. Electrophoresis 14:899-901
Finkelstein, R A; Boesman-Finkelstein, M; Chang, Y et al. (1992) Vibrio cholerae hemagglutinin/protease, colonial variation, virulence, and detachment. Infect Immun 60:472-8
Kazemi, M; Finkelstein, R A (1991) Mapping epitopic regions of cholera toxin B-subunit protein. Mol Immunol 28:865-76
Qu, Z H; Boesman-Finkelstein, M; Kazemi, M et al. (1991) Heterogeneity of immunotypes of heat-labile enterotoxins of enterotoxigenic Escherichia coli of human origin. J Infect Dis 164:796-9
Qu, Z H; Boesman-Finkelstein, M; Finkelstein, R A (1991) Urea-induced release of heat-labile enterotoxin from Escherichia coli. J Clin Microbiol 29:773-7
Boesman-Finkelstein, M; Finkelstein, R A (1991) Bovine lactogenic immunity against pediatric enteropathogens. Adv Exp Med Biol 310:361-7
Kazemi, M; Finkelstein, R A (1991) Identification of epitopes of the receptor binding subunit of cholera toxin by synthetic peptide and CBIB approaches. Adv Exp Med Biol 303:249-54
Hase, C C; Finkelstein, R A (1991) Cloning and nucleotide sequence of the Vibrio cholerae hemagglutinin/protease (HA/protease) gene and construction of an HA/protease-negative strain. J Bacteriol 173:3311-7
Hase, C C; Finkelstein, R A (1990) Comparison of the Vibrio cholerae hemagglutinin/protease and the Pseudomonas aeruginosa elastase. Infect Immun 58:4011-5
Brickman, T J; Boesman-Finkelstein, M; Finkelstein, R A et al. (1990) Molecular cloning and nucleotide sequence analysis of cholera toxin genes of the CtxA- Vibrio cholerae strain Texas Star-SR. Infect Immun 58:4142-4

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