The isolation and examination of P. falciparum mutants will be continued. The long term goals are developing attenuated parasite strains with conditionally lethal, temperature sensitive mutant characters or other types of mutants that affect critical processes involved in parasite growth, parasite-host interactions or contribute to parasite pathogenicity. Testing of temperature sensitive mutants will be done to examine their ability to induce a protective immunity in Aotus trivergatus trivergatus monkeys while producing a benign infection. Mutants will be characterized, to analyze the cellular processes or properties that contribute to the mutant phenotype. Cloning of the P. falciparum genetic material coding for the parasite enzymes dihydrofolate reductase and ornithine decarboxylase will be eventually be done. The development of a system for the in vitro genetic transformation of P. falciparum will be pursued to facilitate further studies of the parasite.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI020437-07
Application #
3565468
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1983-07-01
Project End
1991-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
7
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
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Gu, H M; Inselburg, J (1989) Premature release of Plasmodium falciparum from swollen erythrocytes induced by some antimalarials. J Parasitol 75:153-7

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