Abstract

The long term goal of this work is to identify and characterize P. falciparum erythrocytic stage parasite antigens that are capable of stimulating parasite-inhibitory antibodies. Antigens identified by mouse and human parasite-inhibitory monoclonal antibodies and by monospecific polyclonal antibodies will be used to initially identify parasite antigens. The cloned P. falciparum genetic information (cDNA gDNA) that codes for the antigens of interest will be sequenced to define the antigens structure and to study, if appropriate, the production, regulation and function of an antigen. Appropriate antigens will be produced by cloned genetic information and purified by affinity chromatography techniques. They will be used to immunize Aotus trivergatus monkeys. Those antigens providing a protective-immune response in monkeys will be further examined to define the optimal immunogen for possible use in a vaccine trial that would eventually be conducted in humans.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R22)
Project #
5R22AI022038-06
Application #
3566234
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1985-06-01
Project End
1993-05-31
Budget Start
1990-06-01
Budget End
1991-05-31
Support Year
6
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755

  Publications

Inselburg, J; Bathurst, I C; Kansopon, J et al. (1993) Protective immunity induced in Aotus monkeys by a recombinant SERA protein of Plasmodium falciparum: adjuvant effects on induction of protective immunity. Infect Immun 61:2041-7
Inselburg, J; Bathurst, I C; Kansopon, J et al. (1993) Protective immunity induced in Aotus monkeys by a recombinant SERA protein of Plasmodium falciparum: further studies using SERA 1 and MF75.2 adjuvant. Infect Immun 61:2048-52
Fox, B A; Li, W B; Tanaka, M et al. (1993) Molecular characterization of the largest subunit of Plasmodium falciparum RNA polymerase I. Mol Biochem Parasitol 61:37-48
Inselburg, J; Bzik, D J; Li, W B et al. (1991) Protective immunity induced in Aotus monkeys by recombinant SERA proteins of Plasmodium falciparum. Infect Immun 59:1247-50
Barr, P J; Inselburg, J; Green, K M et al. (1991) Immunogenicity of recombinant Plasmodium falciparum SERA proteins in rodents. Mol Biochem Parasitol 45:159-70
Tanaka, M; Gu, H M; Bzik, D J et al. (1990) Mutant dihydrofolate reductase-thymidylate synthase genes in pyrimethamine-resistant Plasmodium falciparum with polymorphic chromosome duplications. Mol Biochem Parasitol 42:83-91
Gu, H; Inselburg, J W; Bzik, D J et al. (1990) Plasmodium falciparum: analysis of chromosomes separated by contour-clamped homogenous electric fields. Exp Parasitol 71:189-98
Li, W B; Bzik, D J; Horii, T et al. (1989) Structure and expression of the Plasmodium falciparum SERA gene. Mol Biochem Parasitol 33:13-25
Horii, T; Bzik, D J; Inselburg, J (1988) Characterization of antigen-expressing Plasmodium falciparum cDNA clones that are reactive with parasite inhibitory antibodies. Mol Biochem Parasitol 30:9-18
Bzik, D J; Li, W B; Horii, T et al. (1988) Amino acid sequence of the serine-repeat antigen (SERA) of Plasmodium falciparum determined from cloned cDNA. Mol Biochem Parasitol 30:279-88

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