Abstract

The long-range goals include elucidation of control mechanisms of gastrointestinal endocrine cell growth and replication. A further goal includes understanding of abnormal physiological or disease states characterized by disturbances in gastrointestinal endocrine function. This study will examine mechanisms of postvagotomy gastrin cell proliferation.
The specific aims are 1) investigation of the relationship between luminal pH and acute postvagotomy hypergastrinemia, 2) study of the relationships of circulating and tissue somatostatin and gastrin production and release early after vagotomy, 3) study of the time course and mechanisms of denervation-caused gastric endocrine cell proliferation, and 4) identification of changes in gastrin and somatostatin secretory responses to known secretagogues during proliferation and after hyperplastic adaptation. The rat will be the experimental animal. The effects of variations in luminal pH on gastrin release after vagotomy will be studied using an in vivo luminal gastric perfusion model. Investigations of somatostatin cell and gastrin cell responses to various secretagogues will employ an in vivo vascular gastric infusion system. Measurements of tissue hormone content, quantitation of DNA, RNA, and protein production, and estimation of mitotic activity by autoradiography will be used to study the mechanisms of gastric endocrine cell proliferation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Unknown (R23)
Project #
5R23DK037393-02
Application #
3447520
Study Section
Surgery and Bioengineering Study Section (SB)
Project Start
1986-08-01
Project End
1989-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195