The long-term objective is to obtain a better understanding of the structural basis for information processing in the vertebrate retina. The ganglion cells provide an ideal opportunity for studies which correlate structure with function because of their ideal location and easy accessibility for both morphological and physiological analysis. The study will combine use of a newly-developed monoclonal antibody (AB5), which is specific for ganglion cells, with a variety of anatomical techniques to study the structure and synaptic relationships of retinal ganglion cells in both the adult and developing rabbit.
Specific aims for the proposed 3 year study include: 1) A light and electron microscopical immunocytochemical analysis to characterize AB5-labelling of ganglion cells and to study the structure and intraretinal synaptic relationships of the AB5-labelled ganglion cells. 2) A light and electron microscopical immunocytochemical analysis to characterize AB5-labelling of ganglion cells in the developing retina and to study the development of the synaptic relationships of the AB5-labelled ganglion cells. 3) A double-label electron microscopical study combining the techniques of AB5 immunocytochemistry and autoradiography to study the neurotransmitter-specific input to the rabbit retinal ganglion cells. The conventional neurotransmitter candidates GABA and glycine will be investigated. 4) A double-label electron microscopical study combining the techniques of AB5 immunocytochemistry and autoradiography to study the development of neurotransmitter-specific synaptic input to the rabbit retinal ganglion cells.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Unknown (R23)
Project #
1R23EY006469-01
Application #
3447826
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1986-09-30
Project End
1987-09-29
Budget Start
1986-09-30
Budget End
1987-09-29
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030